Department of Medical Science, Graduate School of East-West Medical Science, Kyung Hee University Global Campus, Giheung-Gu, Gyeonggi-Do, South Korea.
Int J Dermatol. 2010 Mar;49(3):276-82. doi: 10.1111/j.1365-4632.2009.04269.x.
Punica granatum (pomegranate) is kind of a fruit consumed fresh or in beverage. It has been widely used in traditional medicine in various parts of the world. In this study, we examined the efficacy of a Punica granatum (PG) extract in protecting skin against UVB-induced damage using cultured human skin fibroblasts.
A Korean red PG sample was used, and its effects classified according to if the PG source originated from the rind, seed and fruit. The polyphenol content of PG, which is known to prevent other adverse cutaneous effects of UV irradiation, was measured by GC-MS. The protective effects of PG on UVB-induced skin photoaging were examined by determining the level of procollagen type I and MMP-1 after UVB irradiation.
Based on the GC-MS quantitative analysis, catechin, quercetin, kaempferol, and equol were the predominant compounds detected in PG. In the changes of expression of procollagen type I and MMP-1 in UV irradiated human skin fibroblasts treated PG, especially extract prepared from rind, the synthesis of collagen was increased and the expression of MMP-1 was decreased.
The major polyphenols in PG, particularly catechin, play a significant role in its photoprotective effects on UVB-induced skin damage.
石榴（pomegranate）是一种可鲜食或制成饮料的水果。它在世界许多地方的传统医学中被广泛应用。在这项研究中，我们使用培养的人皮肤成纤维细胞，研究了石榴提取物（PG）在保护皮肤免受 UVB 损伤方面的功效。
使用了一种韩国红石榴 PG 样本，并根据 PG 的来源（果皮、种子和果实）对其功效进行了分类。通过 GC-MS 测量了 PG 的多酚含量，已知多酚可以预防 UV 照射对皮肤的其他不良影响。通过测定 UVB 照射后 I 型前胶原和 MMP-1 的水平，研究了 PG 对 UVB 诱导的皮肤光老化的保护作用。
基于 GC-MS 的定量分析，在 PG 中检测到的主要化合物有儿茶素、槲皮素、山奈酚和雌马酚。在 PG 处理的 UV 照射人皮肤成纤维细胞中 I 型前胶原和 MMP-1 表达的变化中，特别是来自果皮的提取物，胶原的合成增加，MMP-1 的表达减少。
PG 中的主要多酚，特别是儿茶素，在其对 UVB 诱导的皮肤损伤的光保护作用中发挥了重要作用。
