Anker Jeffrey N, Hall W Paige, Lambert Mary P, Velasco Pauline T, Mrksich Milan, Klein William L, Van Duyne Richard P
Department of Chemistry, Northwestern University, 214x5 Sheridan Road, Evanston, IL 60208, USA.. ; Tel: 747-491-3516;
J Phys Chem C Nanomater Interfaces. 2009 Mar 24;113(15):5891-5894. doi: 10.1021/jp900266k.
High resolution localized surface plasmon resonance (HR-LSPR) sensors were combined with matrix assisted laser desorption ionization mass spectrometry (MALDI-MS) for the first time. LSPR sensors provide real-time label-free detection of molecular adsorption. Subsequent MALDI-MS analysis enables identification of the adsorbed molecules. This synergistic LSPR-MS approach was applied to the detection and identification of amyloid beta oligomers which play an important role in the molecular pathogenesis of Alzheimer's Disease.
高分辨率局域表面等离子体共振（HR-LSPR）传感器首次与基质辅助激光解吸电离质谱（MALDI-MS）相结合。LSPR传感器可对分子吸附进行实时无标记检测。随后的MALDI-MS分析能够识别吸附的分子。这种协同的LSPR-MS方法被应用于检测和识别在阿尔茨海默病分子发病机制中起重要作用的β淀粉样蛋白寡聚体。
