Sørensen Thomas J, Laursen Bo W, Luchowski Rafal, Shtoyko Tanya, Akopova Irina, Gryczynski Zygmunt, Gryczynski Ignacy
Center for Commercialization of Fluorescence Technologies, University of North Texas Health Science Center, Fort Worth, TX 76107, USA.
Chem Phys Lett. 2009 Jul 1;476(1):46-50. doi: 10.1016/j.cplett.2009.05.064.
We report a multi-fold enhancement of the fluorescence of methyl-azadioxatriangulenium chloride (Me-ADOTA*Cl) in PVA deposited on a 50 nm thick gold mirror carrying an evaporation induced self-assembly of colloidal silver nanoparticles (Ag-SACs). The average measured increase in fluorescence emission of about 50-fold is accompanied by hot spots with a local enhancement in brigthness close to 200. The long lifetime of the dye allows for the first direct determination of the correlation between the enhancement of emission intensity and the decrease in fluorescence lifetime. The Ag-SACs surface preparation and observed enhancements are highly reproducible. We believe that these robust plasmonic surfaces will find use in sensing platforms for ultrasensitive detection.
我们报道了在沉积于50纳米厚金镜上的聚乙烯醇（PVA）中，甲基氮杂二氧杂三角烯氯化物（Me-ADOTA*Cl）的荧光增强了数倍，该金镜承载着通过蒸发诱导自组装形成的胶体银纳米颗粒（Ag-SACs）。平均测量到的荧光发射增强约50倍，同时存在热点，其亮度局部增强接近200倍。染料的长寿命使得首次能够直接确定发射强度增强与荧光寿命缩短之间的相关性。Ag-SACs的表面制备以及观察到的增强效果具有高度可重复性。我们相信，这些坚固的等离子体表面将在超灵敏检测的传感平台中得到应用。
