Shtoyko Tanya, Raut Sangram, Rich Ryan M, Sronce Randy J, Fudala Rafal, Mason Rachel N, Akopova Irina, Gryczynski Zygmunt, Gryczynski Ignacy
Department of Chemistry, The University of Texas at Tyler , 3900 University Boulevard, Tyler, Texas 75799, United States.
ACS Appl Mater Interfaces. 2014;6(21):18780-7. doi: 10.1021/am504431j. Epub 2014 Oct 22.
In this report we describe a preparation of silver wires (SWs) on gold mirrors and its application to surface enhanced fluorescence (SEF) using a new methodology. Silica protected gold mirrors were drop-coated with a solution of silver triangular nanoprisms. The triangular nanoprisms were slowly air-dried to get silver wires that self-assembled on the gold mirrors. Fluorescence enhancement was studied using methyl azadioxatriangulenium chloride (Me-ADOTA · Cl) dye in PVA spin-coated on a clean glass coverslip. New Plasmonic Platforms (PPs) were assembled by placing a mirror with SWs in contact with a glass coverslip spin-coated with a uniform Me-ADOTA · Cl film. It was shown that surface enhanced fluorescence is a real phenomenon, not just an enhancement of the fluorescence signal due to an accumulation of the fluorophore on rough nanostructure surfaces. The average fluorescence enhancement was found to be about 15-fold. The lifetime of Me-ADOTA · Cl dye was significantly reduced (∼ 4 times) in the presence of SWs. Moreover, fluorescence enhancement and lifetime did not show any dependence on the excitation light polarization.
在本报告中，我们描述了一种在金镜上制备银线（SWs）的方法及其使用新方法在表面增强荧光（SEF）中的应用。用二氧化硅保护的金镜用银三角纳米棱镜溶液进行滴涂。将三角纳米棱镜缓慢风干以获得自组装在金镜上的银线。使用旋涂在干净玻璃盖玻片上的聚乙烯醇（PVA）中的甲基氮杂二氧杂三嗪鎓氯化物（Me-ADOTA·Cl）染料研究荧光增强。通过将带有SWs的镜子与旋涂有均匀Me-ADOTA·Cl膜的玻璃盖玻片接触来组装新的等离子体平台（PPs）。结果表明，表面增强荧光是一种真实现象，而不仅仅是由于荧光团在粗糙纳米结构表面上的积累而导致的荧光信号增强。发现平均荧光增强约为15倍。在存在SWs的情况下，Me-ADOTA·Cl染料的寿命显著缩短（约4倍）。此外，荧光增强和寿命对激发光偏振没有任何依赖性。
