Robinson J C, Kaufman P L
Department of Ophthalmology, University of Wisconsin School of Medicine, Madison 53792.
Invest Ophthalmol Vis Sci. 1991 Apr;32(5):1614-8.
Total outflow facility was determined in both eyes of cynomolgus monkeys by two-level constant pressure perfusion. After a baseline measurement, one eye of the experimental animal received intracameral cytochalasin B (CB); the other eye received vehicle without CB. Twenty minutes later both eyes received intracameral epinephrine (EPI), and 30 min later the outflow facility was again determined. Doses of CB and EPI which were subthreshold when given singly produced significant facility increases when given concurrently. Subthreshold doses of CB given concurrently with a maximal dose of EPI produced significantly larger facility increases than a maximal dose of EPI given alone. A maximal dose of CB produced a larger facility increase than a maximal dose of EPI. Maximal doses of CB and EPI given concurrently produced a larger facility increase than did a maximal dose of EPI alone and had about the same effect as a maximal CB dose given alone. Since CB's facility-increasing effect is likely due to "disruption" of actin filaments in the endothelial cells of the trabecular meshwork and Schlemm's canal and since CB potentiates the facility-increasing effect of EPI, EPI may also act by "disrupting" actin filaments and consequently altering endothelial cell shape.
通过两级恒压灌注法测定了食蟹猴双眼的总房水流出率。在进行基线测量后，实验动物的一只眼睛前房内注射细胞松弛素B（CB）；另一只眼睛注射不含CB的赋形剂。20分钟后，双眼均前房内注射肾上腺素（EPI），30分钟后再次测定房水流出率。单独使用时阈下剂量的CB和EPI同时给药时可显著提高房水流出率。阈下剂量的CB与最大剂量的EPI同时给药比单独给予最大剂量的EPI能显著提高更大的房水流出率。最大剂量的CB比最大剂量的EPI能产生更大的房水流出率增加。最大剂量的CB和EPI同时给药比单独给予最大剂量的EPI能产生更大的房水流出率增加，且与单独给予最大剂量的CB效果大致相同。由于CB增加房水流出率的作用可能是由于小梁网和施莱姆管内皮细胞中肌动蛋白丝的“破坏”，且CB能增强EPI增加房水流出率的作用，所以EPI可能也通过“破坏”肌动蛋白丝从而改变内皮细胞形状来发挥作用。
