Kaufman P L, Erickson K A
Invest Ophthalmol Vis Sci. 1982 Nov;23(5):646-50.
Total outflow facility was determined by two-level constant-pressure perfusion in cynomolgus monkeys for 30 to 40 min before and after intracameral infusion of cytochalasins B and D (CB, CD). CB and CD dose-facility response relationships were defined. CB doses of 0.1, 0.2, and 0.5 micrograms were subthreshold; 1, 2, 5 and 10 micrograms were effective, with 5 micrograms probably supramaximal. CD at 0.02 microgram was probably subthreshold; 0.1, 0.5, 1, 2.5, and 5 micrograms doses were effective, with 0.1 microgram being near or above maximum. Although the maximum effects of both agents were quantitatively similar, approximately doubling-starting facility, CD Was about 25 times more potent. Therefore, CB- and CD-induced facility increases are presumably due largely to interference with formation, structure, and function of actin filaments in the endothelial cells of the trabecular meshwork and inner wall of Schlemm's canal.
通过对食蟹猴进行两级恒压灌注来测定房水总流出率，在向前房内注入细胞松弛素B和D（CB、CD）之前和之后分别持续30至40分钟。确定了CB和CD的剂量-流出率反应关系。CB剂量为0.1、0.2和0.5微克时为阈下剂量；1、2、5和10微克有效，其中5微克可能为超最大剂量。CD剂量为0.02微克时可能为阈下剂量；0.1、0.5、1、2.5和5微克有效，其中0.1微克接近或高于最大剂量。尽管两种药物的最大效应在数量上相似，起始流出率约增加一倍，但CD的效力约为CB的25倍。因此，CB和CD引起的流出率增加可能主要是由于干扰了小梁网和施莱姆管内壁内皮细胞中肌动蛋白丝的形成、结构和功能。
