Robinson J C, Kaufman P L
Department of Ophthalmology and Visual Sciences, University of Wisconsin, Madison.
Arch Ophthalmol. 1994 Dec;112(12):1610-3. doi: 10.1001/archopht.1994.01090240116035.
To determine whether phalloidin, a fungal peptide that inhibits actin filament depolymerization, could inhibit the ability of cytochalasin B and epinephrine to increase the facility of aqueous outflow in the eyes of living cynomolgus monkeys.
Outflow facility was determined by two-level constant-pressure perfusion of the anterior chamber. After measurement of baseline facility in both eyes, one eye of each animal received intracameral phalloidin (1.3, 13, or 130 mumol/L); the opposite eye received vehicle. Both eyes then received either epinephrine (0.3 mmol/L) or cytochalasin B (0.2 mmol/L), and facility was again measured.
Cytochalasin B and epinephrine increased facility by 120% to 190% and 100% to 180%, respectively (uncorrected for 15% resistance washout caused by perfusion itself). Phalloidin itself (13 or 130 mumol/L) did not affect facility, but it inhibited up to 50% of the facility-increasing effect of cytochalasin B and epinephrine.
We conclude that (1) the aqueous humor outflow facilitating effects of cytochalasin B or epinephrine depend in some manner on depolymerization of actin filaments within trabecular meshwork cells, and (2) actin filaments may help regulate aqueous outflow.
确定抑制肌动蛋白丝解聚的真菌肽鬼笔环肽是否能抑制细胞松弛素B和肾上腺素增加恒河猴活体眼睛房水流出易度的能力。
通过前房两级恒压灌注来确定流出易度。在测量双眼的基线易度后，每只动物的一只眼睛接受前房内注射鬼笔环肽（1.3、13或130 μmol/L）；对侧眼睛接受赋形剂。然后双眼都接受肾上腺素（0.3 mmol/L）或细胞松弛素B（0.2 mmol/L），并再次测量易度。
细胞松弛素B和肾上腺素分别使易度增加120%至190%和100%至180%（未校正灌注本身导致的15%阻力消除）。鬼笔环肽本身（13或130 μmol/L）不影响易度，但它可抑制细胞松弛素B和肾上腺素高达50%的易度增加效应。
我们得出结论：（1）细胞松弛素B或肾上腺素促进房水流出的作用在某种程度上取决于小梁网细胞内肌动蛋白丝的解聚，且（2）肌动蛋白丝可能有助于调节房水流出。
