Roy Rabindra N, Roy Lakshmi N, Fuge Michael S, Roy Chandra N, Himes Curtis A, Bryant Paul A, Robinson Kyle T, Kaufmann Daniel A, Grove Colby H, Ghosh Toshi, Bwashi Adonis
J Solution Chem. 2009 Apr 1;38(4):471-483. doi: 10.1007/s10953-009-9380-9.
The values of the second dissociation constant p and related thermodynamic quantities of [N-(2-acetamido)-2-aminoethanesulfonic acid] (ACES) have already been reported over the temperature range 5 to 55°C including 37°C. This paper reports the pa values of four chloride ion free buffer solutions and eight buffer solutions with = 0.16 mol·kg , matching closely to that of the physiological sample. Conventional pa values for all twelve buffer solutions from 5 to 55°C, are reported. The residual liquid junction potential correction for two widely used temperatures, 25 and 37°C, has been made. The flowing-junction calomel cell method has been utilized to measure , the liquid junction potential. The operational pH values for four buffer solutions at 25 and 37°C are calculated using the physiological phosphate buffer standard based on NBS/NIST convention. These solutions are recommended as pH standards in the pH range of 6.8 to 7.2 for physiological fluids.
N-(2-乙酰氨基)-2-氨基乙烷磺酸的第二解离常数p值及相关热力学量已在5至55°C（包括37°C）的温度范围内报道。本文报告了四种无氯离子缓冲溶液和八种离子强度I = 0.16 mol·kg⁻¹（与生理样品的离子强度非常接近）的缓冲溶液的pKa值。报道了所有十二种缓冲溶液在5至55°C的常规pKa值。对两个广泛使用的温度25°C和37°C进行了残余液接电位校正。采用流动接界甘汞电池法测量液接电位Ej。基于NBS/NIST惯例，使用生理磷酸盐缓冲标准计算了四种缓冲溶液在25°C和37°C时的操作pH值。这些溶液被推荐作为生理流体在6.8至7.2 pH范围内的pH标准。
