Roy Rabindra N, Roy Lakshmi N, Stegner Jessica M, Sechler Shay A, Jenkins Alexis L, Krishchenko Roman, Henson Isaac B
Hoffman Department of Chemistry, Drury University, 900 N. Benton Ave., Springfield, MO 65802, USA.
J Electroanal Chem (Lausanne). 2011 Dec 1;663(1):8-13. doi: 10.1016/j.jelechem.2011.09.019.
This paper reports the results for the pH of six buffer solutions free of chloride ion with compositions: (a) BES (0.03 mol·kg(-1)) + NaBES (0.09 mol·kg(-1)); (b) BES (0.02 mol·kg(-1)) + NaBES (0.04 mol·kg(-1)); (c) BES (0.04 mol·kg(-1)) + NaBES (0.08 mol·kg(-1)); (d) BES (0.04 mol·kg(-1)) + NaBES (0.04 mol·kg(-1)) (e) BES (0.05 mol·kg(-1)) + NaBES (0.05 mol·kg(-1)); and (f) (0.06 mol·kg(-1)) + NaBES (0.06 mol·kg(-1)). The remaining eight buffer solutions (g) to (n) have saline media of the ionic strength I = 0.16 mol·kg(-1), matching closely to that of the physiological sample. Conventional pa(H) values, designated as pH(s), for all six buffer solutions (a) - (f) without the chloride ion and eight buffer solutions with the chloride ion (g) - (n) at I = 0.16 mol·kg(-1) from (278.15 K to 328.15) K have been calculated. The operational pH values for five buffer solutions at T = 298.15 K and T = 310.15 K have been determined based on the difference in the values of the liquid junction potentials between the blood phosphate standard and the experimental buffer solutions. Five of these buffers are recommended as secondary standards for the physiological pH range 7.5 to 8.5.
本文报道了六种无氯离子缓冲溶液的pH值结果，其组成如下：(a) BES（0.03 mol·kg⁻¹）+ NaBES（0.09 mol·kg⁻¹）；(b) BES（0.02 mol·kg⁻¹）+ NaBES（0.04 mol·kg⁻¹）；(c) BES（0.04 mol·kg⁻¹）+ NaBES（0.08 mol·kg⁻¹）；(d) BES（0.04 mol·kg⁻¹）+ NaBES（0.04 mol·kg⁻¹）；(e) BES（0.05 mol·kg⁻¹）+ NaBES（0.05 mol·kg⁻¹）；以及(f) BES（0.06 mol·kg⁻¹）+ NaBES（0.06 mol·kg⁻¹）。其余八种缓冲溶液(g)至(n)含有离子强度I = 0.16 mol·kg⁻¹的盐溶液介质，与生理样品的离子强度非常接近。已计算出所有六种无氯离子的缓冲溶液(a) - (f)以及八种含有氯离子且离子强度I = 0.16 mol·kg⁻¹的缓冲溶液(g) - (n)在(278.15 K至328.15) K下的常规pa(H)值，记为pH(s)。基于血液磷酸盐标准溶液与实验缓冲溶液之间液接电位值的差异，已确定了五种缓冲溶液在T = 298.15 K和T = 310.15 K时的操作pH值。其中五种缓冲溶液被推荐作为生理pH范围7.5至8.5的二级标准。
