罗勒不同提取物的抗菌活性及利用扫描电子显微镜观察其对细菌细胞的抑制作用
Antimicrobial activity of various extracts of Ocimum basilicum L. and observation of the inhibition effect on bacterial cells by use of scanning electron microscopy.
作者信息
Kaya Ilhan, Yigit Nazife, Benli Mehlika
机构信息
Yuzuncuyil University, Faculty of Agriculture, Department of Plant Protection, Van, Turkey.
出版信息
Afr J Tradit Complement Altern Med. 2008 Jun 18;5(4):363-9. doi: 10.4314/ajtcam.v5i4.31291.
Abstract
The antimicrobial activities of chloroform, acetone and two different concentrations of methanol extracts of Ocimum basilicum L. were studied. These extracts were tested in vitro against 10 bacteria and 4 yeasts strains by the disc diffusion method. The results indicated that the methanol extracts of O. basilucum exhibited the antimicrobial activity against tested microorganisms. While the chloroform and acetone extracts had no effect, the methanol extracts showed inhibition zones against strains of Pseudomonas aeruginosa, Shigella sp., Listeria monocytogenes, Staphylococcus aureus and two different strains of Escherichia coli. The cells of microorganisms, which were treated and untreated with plant extracts, were observed by using the scanning electron microscope. It was observed that the treated cells were damaged.
摘要
研究了罗勒叶的氯仿、丙酮提取物以及两种不同浓度甲醇提取物的抗菌活性。采用纸片扩散法对这些提取物进行体外测试，以检测其对10种细菌菌株和4种酵母菌株的抗菌活性。结果表明，罗勒叶甲醇提取物对测试微生物具有抗菌活性。氯仿提取物和丙酮提取物无抗菌效果，而甲醇提取物对铜绿假单胞菌、志贺氏菌属、单核细胞增生李斯特菌、金黄色葡萄球菌以及两种不同的大肠杆菌菌株表现出抑菌圈。使用扫描电子显微镜观察经植物提取物处理和未处理的微生物细胞。结果发现，经处理的细胞受到了损伤。
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