Muthulakshmi Lakshmanan, Vijayakumar T, Selvam P, Annaraj J, Ranjan Shivendu, Dasgupta Nandita
Department of Biotechnology, Kalasalingam Academy of Research and Education, Krishnankoil, 626 126 India.
Department of Materials Science, Madurai Kamaraj University, Palkalai Nagar, Madurai, Tamil Nadu 625 021 India.
3 Biotech. 2021 Apr;11(4):153. doi: 10.1007/s13205-021-02687-x. Epub 2021 Mar 3.
In this study, (a proven broad spectrum medicinal plant for broad-spectrum pharmacological activities) leaf extract was used as conjugates for the fabrication of silver nanoparticles (AgNP). Color change of the reaction mixture and UV-Visible spectrophotometry indicated the fabrication of silver nanoparticles, further X-ray diffraction (XRD) crystallography, scanning electron microscopy (SEM), transmission electron microscopic images (TEM), and Selected area electron diffraction (SAED) confirms the purity, monodispersity, and morphology including size (22.4 nm) and conjugated functional group of . The conjugation of functional OH, N-O, and C=O groups was confirmed by Fourier-transform infrared spectroscopy (FT-IR). The engineered AgNP have shown significantly efficient antibacterial and antibiofilm activities (92.7% biofilm inhibition) on diverse clinical strains and thus showed its potential for use in clinical applications.
在本研究中，（一种具有广谱药理活性的经证实的广谱药用植物）叶提取物被用作制备银纳米颗粒（AgNP）的共轭物。反应混合物的颜色变化和紫外可见分光光度法表明银纳米颗粒的制备，进一步的X射线衍射（XRD）晶体学、扫描电子显微镜（SEM）、透射电子显微镜图像（TEM）和选区电子衍射（SAED）证实了其纯度、单分散性以及形态，包括尺寸（22.4纳米）和的共轭官能团。通过傅里叶变换红外光谱（FT-IR）证实了官能团OH、N-O和C=O的共轭。工程化的AgNP对多种临床菌株显示出显著高效的抗菌和抗生物膜活性（生物膜抑制率达92.7%），因此显示出其在临床应用中的潜力。
