Adewole Stephen O, Ojewole John A O
Department of Pharmacology, School of Pharmacy & Pharmacology, Faculty of Health Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa.
Afr J Tradit Complement Altern Med. 2008 Oct 25;6(1):30-41. doi: 10.4314/ajtcam.v6i1.57071.
Extracts from various morphological parts of Annona muricata Linn. (Annonaceae) are widely used medicinally in many parts of the world for the management, control and/or treatment of a plethora of human ailments, including diabetes mellitus (DM). The present study was undertaken to investigate the possible protective effects of A. muricata leaf aqueous extract (AME) in rat experimental paradigms of DM. The animals used were broadly divided into four (A, B, C and D) experimental groups. Group A rats served as 'control' animals and received distilled water in quantities equivalent to the administered volumes of AME and reference drugs' solutions intraperitoneally. Diabetes mellitus was induced in Groups B and C rats by intraperitoneal injections of streptozotocin (STZ, 70 mg kg(-1)). Group C rats were additionally treated with AME (100 mg kg(-1) day(-1), p.o.) as from day 3 post STZ injection, for four consecutive weeks. Group D rats received AME (100 mg kg(-1) day(-1) p.o.) only for four weeks. Post-euthanization, hepatic tissues were excised and processed biochemically for antioxidant enzymes and lipid profiles, such as catalase (CAT), reactive oxygen species (ROS), glutathione (GSH), superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), thiobarbituric acid reactive substances (TBARS), triglycerides (TG), total cholesterol (TC), high density lipoprotein (HDL) and low density lipoprotein (LDL), respectively. Treatment of Groups B and C rats with STZ (70 mg kg(-1) i. p.) resulted in hyperglycaemia, hypoinsulinaemia, and increased TBARS, ROS, TC, TG and LDL levels. STZ treatment also significantly decreased (p<0.05) CAT, GSH, SOD, GSH-Px activities, and HDL levels. AME-treated Groups C and D rats showed significant decrease (p<0.05) in elevated blood glucose, ROS, TBARS, TC, TG and LDL. Furthermore, AME treatment significantly increased (p<0.05) antioxidant enzymes' activities, as well as serum insulin levels. The findings of this laboratory animal study suggest that A. muricata extract has a protective, beneficial effect on hepatic tissues subjected to STZ-induced oxidative stress, possibly by decreasing lipid peroxidation and indirectly enhancing production of insulin and endogenous antioxidants.
刺果番荔枝（番荔枝科）不同形态部位的提取物在世界许多地区被广泛用于管理、控制和/或治疗多种人类疾病，包括糖尿病（DM）。本研究旨在探讨刺果番荔枝叶水提取物（AME）在大鼠糖尿病实验模型中的可能保护作用。所用动物大致分为四个（A、B、C和D）实验组。A组大鼠作为“对照”动物，腹腔注射相当于AME和参比药物溶液给药体积的蒸馏水。B组和C组大鼠通过腹腔注射链脲佐菌素（STZ，70 mg kg⁻¹）诱导糖尿病。C组大鼠从STZ注射后第3天起额外用AME（100 mg kg⁻¹ 天⁻¹，口服）连续治疗四周。D组大鼠仅接受AME（100 mg kg⁻¹ 天⁻¹，口服）四周。处死后，切除肝脏组织并进行生化处理以检测抗氧化酶和脂质谱，分别如过氧化氢酶（CAT）、活性氧（ROS）、谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）、硫代巴比妥酸反应性物质（TBARS）、甘油三酯（TG）、总胆固醇（TC）、高密度脂蛋白（HDL）和低密度脂蛋白（LDL）。用STZ（70 mg kg⁻¹，腹腔注射）处理B组和C组大鼠导致高血糖、低胰岛素血症，并使TBARS、ROS、TC、TG和LDL水平升高。STZ处理还显著降低（p<0.05）CAT、GSH、SOD、GSH-Px活性以及HDL水平。AME处理的C组和D组大鼠的血糖、ROS、TBARS、TC、TG和LDL升高水平显著降低（p<0.05）。此外，AME处理显著提高（p<0.05）抗氧化酶活性以及血清胰岛素水平。这项实验动物研究的结果表明，刺果番荔枝提取物对遭受STZ诱导的氧化应激的肝脏组织具有保护、有益作用，可能是通过减少脂质过氧化并间接增强胰岛素和内源性抗氧化剂的产生。
