Key Laboratory of Modern Chinese Medicines (China Pharmaceutical University), Ministry of Education, Nanjing, P. R. China.
J Sep Sci. 2010 Mar;33(4-5):570-81. doi: 10.1002/jssc.200900687.
A structurally identified new compound named malonylastragaloside I was isolated and obtained from Radix Astragali. This novel compound was found to be unstable especially under high temperature and pH value. Using sonication extraction, addition of formic acid, and an efficient medium pressure ODS C(18) column chromatography method, a high yield of 40 mg of this compound was obtained from 150 g of powdered crude herbal medicine. Malonylastragaloside I was structurally characterized by NMR and ESI quadrupole TOF MS. With the strategy of target precursor ions scan, a total of 22 astragalosides including 8 astragaloside malonates were screened and characterized from the methanolic extract of Radix Astragali by HPLC-Q-TOF/MS. The eight astragaloside malonates were found in both Astragalus membranaceus var. mongholicus and A. membranaceus. The results provided a real profile of various triterpene saponins in Radix Astragali. It is a first report regarding isolation and characterization of astragaloside malonates in Astragalus species.
从黄芪中分离得到一种结构明确的新化合物，命名为丙二酰黄芪苷 I。该新化合物在高温和 pH 值条件下特别不稳定。通过超声提取、添加甲酸以及高效中压 ODS C(18)柱色谱法，从 150 克粉末状粗草药中获得了 40 毫克该化合物的高产率。通过 NMR 和 ESI 四极杆飞行时间质谱法对丙二酰黄芪苷 I 的结构进行了表征。通过目标前体离子扫描策略，从黄芪的甲醇提取物中通过 HPLC-Q-TOF/MS 筛选和鉴定了 22 种黄芪苷，包括 8 种黄芪苷丙二酸盐。这 8 种黄芪苷丙二酸盐在蒙古黄芪和膜荚黄芪中均有发现。结果提供了黄芪中各种三萜皂苷的真实图谱。这是首次在黄芪属植物中分离和鉴定黄芪苷丙二酸盐。
