Department of Medical Microbiology and Parasitology, Institutes of Medical Sciences, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Biomed Environ Sci. 2009 Oct;22(5):401-12. doi: 10.1016/S0895-3988(10)60018-9.
To identify the antimicrobial resistance of commercial lactic acid bacteria present in microbial foods and drug additives by analyzing their isolated strains used for fermentation and probiotics.
Antimicrobial susceptibility of 41 screened isolates was tested with disc diffusion and E-test methods after species-level identification. Resistant strains were selected and examined for the presence of resistance genes by PCR.
Distribution of resistance was found in different species. All isolates were susceptible to chloramphenicol, tetracycline, ampicillin, amoxicillin/clavulanic acid, cephalothin, and imipenem. In addition, isolates resistant to vancomycin, rifampicin, streptomycin, bacitracin, and erythromycin were detected, although the incidence of resistance to these antibiotics was relatively low. In contrast, most strains were resistant to ciprofloxacin, amikacin, trimethoprim/sulphamethoxazole, and gentamycin. The genes msrC, vanX, and dfrA were detected in strains of Enterococcus faecium, Lactobacillus plantarum, Streptococcus thermophilus, and Lactococcus lactis.
Antibiotic resistance is present in different species of probiotic strains, which poses a threat to food safety. Evaluation of the safety of lactic acid bacteria for human consumption should be guided by established criteria, guidelines and regulations.
通过分析用于发酵和益生菌的分离菌株，确定微生物食品和药物添加剂中商业乳酸菌的抗菌药物耐药性。
对 41 株筛选出的分离株进行种属水平鉴定后，采用纸片扩散法和 E 试验法进行药敏试验。选择耐药株并通过 PCR 检测耐药基因。
不同种属的分离株均有耐药分布。所有分离株均对氯霉素、四环素、氨苄西林、阿莫西林/克拉维酸、头孢噻吩和亚胺培南敏感。此外，还检测到对万古霉素、利福平、链霉素、杆菌肽和红霉素耐药的分离株，尽管这些抗生素的耐药发生率相对较低。相比之下，大多数菌株对环丙沙星、阿米卡星、复方磺胺甲噁唑和庆大霉素耐药。粪肠球菌、植物乳杆菌、嗜热链球菌和乳球菌中检测到 msrC、vanX 和 dfrA 基因。
益生菌菌株存在不同种属的抗生素耐药性，这对食品安全构成威胁。应根据既定标准、指南和法规评估食用乳酸菌的安全性。
