Temmerman R, Pot B, Huys G, Swings J
Laboratorium voor Microbiologie, Universiteit Gent, K.L. Ledeganckstr. 35, B-9000, Ghent, Belgium.
Int J Food Microbiol. 2003 Feb 25;81(1):1-10. doi: 10.1016/s0168-1605(02)00162-9.
In the present study, a total of 55 European probiotic products were evaluated with regard to the identity and the antibiotic resistance of the bacterial isolates recovered from these products. Bacterial isolation from 30 dried food supplements and 25 dairy products, yielded a total of 268 bacterial isolates selected from several selective media. Counts of food supplements showed bacterial recovery in 19 (63%) of the dried food supplements ranging from 10(3) to 10(6) CFU/g, whereas all dairy products yielded growth in the range of 10(5)-10(9) CFU/ml. After identification of the isolates using whole-cell protein profiling, mislabeling was noted in 47% of the food supplements and 40% of the dairy products. In six food supplements, Enterococcus faecium was isolated whereas only two of those products claim this species on their label. Using the disc diffusion method, antibiotic resistance among 187 isolates was detected against kanamycin (79% of the isolates), vancomycin (65%), tetracycline (26%), penicillinG (23%), erythromycin (16%) and chloramphenicol (11%). Overall, 68.4% of the isolates showed resistance against multiple antibiotics including intrinsic resistances. Initially, 38% of the isolated enterococci was classified as vancomycin resistant using the disc diffusion method, whereas additional broth dilution and PCR assays clearly showed that all E. faecium isolates were in fact vancomycin susceptible.
在本研究中，对55种欧洲益生菌产品进行了评估，以确定从这些产品中分离出的细菌菌株的身份和抗生素抗性。从30种干制食品补充剂和25种乳制品中分离细菌，从几种选择性培养基中总共筛选出268株细菌菌株。食品补充剂的计数显示，19种（63%）干制食品补充剂中有细菌生长，范围为10³至10⁶CFU/g，而所有乳制品的生长范围为10⁵至10⁹CFU/ml。使用全细胞蛋白质谱对分离株进行鉴定后，发现47%的食品补充剂和40%的乳制品存在标签错误。在六种食品补充剂中分离出了粪肠球菌，而这些产品中只有两种在标签上标明了该菌种。使用纸片扩散法，检测了187株分离株对卡那霉素（79%的分离株）、万古霉素（65%）、四环素（26%）、青霉素G（23%）、红霉素（16%）和氯霉素（11%）的抗生素抗性。总体而言，68.4%的分离株对多种抗生素表现出抗性，包括固有抗性。最初，使用纸片扩散法将38%的分离出的肠球菌分类为万古霉素抗性，而进一步的肉汤稀释和PCR检测清楚地表明，所有粪肠球菌分离株实际上对万古霉素敏感。
