活细胞中生物分子的单分子光谱和成像。
Single-molecule spectroscopy and imaging of biomolecules in living cells.
机构信息
Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
出版信息
Anal Chem. 2010 Mar 15;82(6):2192-203. doi: 10.1021/ac9024889.
Abstract
The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a single molecule were completed over two decades ago. Single-molecule spectroscopy has developed into a field that includes a wealth of experiments at room temperature and inside living cells. The fast growth of single-molecule biophysics has resulted from its benefits in probing heterogeneous populations, one molecule at a time, as well as from advances in microscopes and detectors. This Perspective summarizes the field of live-cell imaging of single biomolecules.
摘要
自二十多年前首次成功实现单分子光学检测以来，每年有关单分子成像实验的报告数量呈大致指数级增长。单分子光谱学已发展成为一个领域，其中包括在室温下和活细胞内进行的大量实验。单分子生物物理学的快速发展得益于其在探测异质群体（一次一个分子）方面的优势，以及显微镜和探测器的进步。本观点总结了单生物分子活细胞成像领域。
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本文引用的文献
1
Detectors for single-molecule fluorescence imaging and spectroscopy.
J Mod Opt. 2007 Jan 1;54(2-3):239. doi: 10.1080/09500340600769067.
2
Proposed method for molecular optical imaging.
Opt Lett. 1995 Feb 1;20(3):237-9. doi: 10.1364/ol.20.000237.
3
Single-molecule microscopy reveals membrane microdomain organization of cells in a living vertebrate.
Biophys J. 2009 Aug 19;97(4):1206-14. doi: 10.1016/j.bpj.2009.05.044.
4
Cluster size regulates protein sorting in the immunological synapse.
Proc Natl Acad Sci U S A. 2009 Aug 4;106(31):12729-34. doi: 10.1073/pnas.0902621106. Epub 2009 Jul 21.
5
GFP: lighting up life (Nobel Lecture).
Angew Chem Int Ed Engl. 2009;48(31):5603-11. doi: 10.1002/anie.200902040.
6
Advances in light microscopy for neuroscience.
Annu Rev Neurosci. 2009;32:435-506. doi: 10.1146/annurev.neuro.051508.135540.
7
Non-blinking and photostable upconverted luminescence from single lanthanide-doped nanocrystals.
Proc Natl Acad Sci U S A. 2009 Jul 7;106(27):10917-21. doi: 10.1073/pnas.0904792106. Epub 2009 Jun 18.
8
Velocity, processivity, and individual steps of single myosin V molecules in live cells.
Biophys J. 2009 May 20;96(10):4268-75. doi: 10.1016/j.bpj.2009.02.045.
9
Super-resolution video microscopy of live cells by structured illumination.
Nat Methods. 2009 May;6(5):339-42. doi: 10.1038/nmeth.1324. Epub 2009 Apr 26.
10
Single-chromophore-based photoswitchable nanoparticles enable dual-alternating-color fluorescence for unambiguous live cell imaging.
J Am Chem Soc. 2009 Apr 1;131(12):4245-52. doi: 10.1021/ja805150g.
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