Department of Chemistry, Stanford University, Stanford, California 94305-5080, USA.
Anal Chem. 2010 Mar 15;82(6):2192-203. doi: 10.1021/ac9024889.
The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a single molecule were completed over two decades ago. Single-molecule spectroscopy has developed into a field that includes a wealth of experiments at room temperature and inside living cells. The fast growth of single-molecule biophysics has resulted from its benefits in probing heterogeneous populations, one molecule at a time, as well as from advances in microscopes and detectors. This Perspective summarizes the field of live-cell imaging of single biomolecules.
自二十多年前首次成功实现单分子光学检测以来，每年有关单分子成像实验的报告数量呈大致指数级增长。单分子光谱学已发展成为一个领域，其中包括在室温下和活细胞内进行的大量实验。单分子生物物理学的快速发展得益于其在探测异质群体（一次一个分子）方面的优势，以及显微镜和探测器的进步。本观点总结了单生物分子活细胞成像领域。
