Postgraduate Program Students, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
Department of Internal Disease, Faculty of Medicine, Brawijaya University, Malang, Indonesia.
Open Vet J. 2023 Jan;13(1):11-19. doi: 10.5455/OVJ.2023.v13.i1.2. Epub 2023 Jan 5.
Particulate matter (PM) is one of the important components in air pollution that can cause endothelial vascular dysfunction through exacerbation of atherosclerosis and inflammation of the respiratory system. Increased levels of malondialdehyde (MDA) in blood plasma can be an indicator of oxidative stress. Then, macrophages can secrete proinflammatory cytokines that will stimulate immune cells and vascular endothelial cells to release inflammatory cytokines, such as interleukin-6 (IL-6) and tumor necrotic factor-α (TNF-α). works by scavenging the active free radicals involved in the peroxidation process.
This study aims to prove that the administration of can reduce MDA, TNF-α, and IL-6 levels in exposed to soot particulates.
The subjects of this study were 30 male rats which were divided into 5 treatment groups with the following: (C0): negative control; (C+): positive control; (T1): Treatment group 2, rats exposed to particulate soot at a concentration of 1,064 mg/m for 8 hours and given at a dose of 1 mg/kg bw; (T2): Treatment group 3 was rats exposed to soot particulates at a concentration of 1,064 mg/m for 8 hours and given at a dose of 2 mg/kg bw; (T3): Treatment group 4 was rats exposed to soot particulates at a concentration of 1,064 mg/m for 8 hours and given at a dose of 3 mg/kg bw.Giving the extract orally with a probe every day for 30 days after treatment of exposure to soot. Examination of MDA, TNF-, and IL-6 levels with the ELISA method.
The administration of can reduce MDA while the lowest MDA levels were obtained in the T3 treatment with an average of 1.542 ± 0.231. The results of the description of the lowest levels of TNF-α were obtained in the C-treatment with an average of 55.981 ± 4.689. Then, the lowest levels of IL-6 were obtained in the C-treatment with an average of 2.292 ± 0.461.
The results stated that the administration of could reduce MDA levels, TNF-α, and IL-6 levels. as an anti-inflammatory and anti-oxidant play an effective role in inhibiting inflammation by decreasing IL-6 cytokine and TNF-α. can inhibit lipid peroxidation initiated by free radicals and then reduce MDA levels.
颗粒物（PM）是空气污染的重要组成部分之一，可通过加剧动脉粥样硬化和呼吸系统炎症导致血管内皮功能障碍。血浆中丙二醛（MDA）水平升高可作为氧化应激的指标。然后，巨噬细胞可以分泌促炎细胞因子，从而刺激免疫细胞和血管内皮细胞释放白细胞介素 6（IL-6）和肿瘤坏死因子-α（TNF-α）等炎症细胞因子。 通过清除参与过氧化过程的活性自由基而起作用。
本研究旨在证明给予 可降低暴露于煤烟颗粒的大鼠 MDA、TNF-α 和 IL-6 水平。
本研究的对象是 30 只雄性大鼠，分为 5 个治疗组，分别为：（C0）：阴性对照；（C+）：阳性对照；（T1）：治疗组 2，大鼠暴露于浓度为 1,064mg/m 的煤烟颗粒 8 小时，给予 1mg/kg bw 剂量；（T2）：治疗组 3 为大鼠暴露于浓度为 1,064mg/m 的煤烟颗粒 8 小时，给予 2mg/kg bw 剂量；（T3）：治疗组 4 为大鼠暴露于浓度为 1,064mg/m 的煤烟颗粒 8 小时，给予 3mg/kg bw 剂量。治疗后，每天用探头口服给予 提取物 30 天。采用 ELISA 法检测 MDA、TNF-α 和 IL-6 水平。
给予 可降低 MDA，而 T3 治疗组 MDA 水平最低，平均为 1.542±0.231。TNF-α 水平最低的描述结果在 C 治疗中获得，平均为 55.981±4.689。然后，在 C 治疗中获得了 IL-6 水平最低，平均为 2.292±0.461。
结果表明，给予 可降低 MDA、TNF-α 和 IL-6 水平。作为一种抗炎和抗氧化剂， 通过降低白细胞介素 6 细胞因子和 TNF-α 发挥抑制炎症的有效作用。 可抑制自由基引发的脂质过氧化反应，从而降低 MDA 水平。
