鉴定心肌肌球蛋白亚基缔合所需的序列。
Identification of sequences necessary for the association of cardiac myosin subunits.
作者信息
McNally E M, Bravo-Zehnder M M, Leinwand L A
机构信息
Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, New York 10461.
出版信息
J Cell Biol. 1991 May;113(3):585-90. doi: 10.1083/jcb.113.3.585.
Abstract
To begin to understand the nature of myosin subunit assembly, we determined the region of a vertebrate sarcomeric myosin heavy chain required for binding of light chain 1. We coexpressed in Escherichia coli segments of the rat alpha cardiac myosin heavy chain which spanned the carboxyl terminus of subfragment 1 and the amino terminus of subfragment 2 with a full-length rat cardiac myosin light chain 1. A 16 amino acid region of the myosin heavy chain (residues 792-808) was shown to be required for myosin light chain 1 binding in an immunoprecipitation assay.
摘要
为了开始理解肌球蛋白亚基组装的本质，我们确定了脊椎动物肌节肌球蛋白重链中与轻链1结合所需的区域。我们在大肠杆菌中共表达了大鼠α心肌肌球蛋白重链的片段，该片段跨越亚片段1的羧基末端和亚片段2的氨基末端，以及全长大鼠心肌肌球蛋白轻链1。在免疫沉淀试验中，肌球蛋白重链的一个16个氨基酸的区域（第792 - 808位氨基酸残基）被证明是肌球蛋白轻链1结合所必需的。
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