两种蚜虫传播的大豆花叶病毒株系外壳蛋白基因的核苷酸序列。

Nucleotide sequences of the coat protein genes of two aphid-transmissible strains of soybean mosaic virus.

作者信息

Jayaram C, Hill J H, Miller W A

机构信息

Department of Plant Pathology, Iowa State University, Ames 50011.

出版信息

J Gen Virol. 1991 Apr;72 ( Pt 4):1001-3. doi: 10.1099/0022-1317-72-4-1001.

DOI:10.1099/0022-1317-72-4-1001

PMID:2016589

Abstract

The nucleotide sequences of the coat protein genes and 3' non-coding regions of two aphid-transmissible isolates (G2 and G7) of soybean mosaic virus (SMV) were determined. The coat protein of the G2 isolate differs from that of the aphid non-transmissible N isolate by a single amino acid at position 12 (aspartic acid in N, glycine in G2 and G7). The G7 isolate differs from G2 and N at three and four amino acid residues, respectively. The nucleotide sequence similarity of the three isolates in the coat protein coding and 3' non-coding regions ranges from 93 to 100%, respectively. In contrast, watermelon mosaic virus 2 (WMV 2) is only 77% to 79% similar to the SMV isolates, suggesting that WMV 2 is a distinct virus and not an isolate of SMV as proposed previously.

摘要

测定了大豆花叶病毒（SMV）两个蚜虫传播分离株（G2和G7）的外壳蛋白基因及3'非编码区的核苷酸序列。G2分离株的外壳蛋白与蚜虫非传播性N分离株的外壳蛋白在第12位氨基酸处存在一个差异（N株为天冬氨酸，G2和G7株为甘氨酸）。G7分离株分别与G2和N株在三个和四个氨基酸残基处存在差异。这三个分离株在外壳蛋白编码区和3'非编码区的核苷酸序列相似性分别为93%至100%。相比之下，西瓜花叶病毒2（WMV 2）与SMV分离株的相似性仅为77%至７9%，这表明WMV 2是一种不同的病毒，而不是先前提出的SMV分离株。