Wang Y Y, Beck D L, Gardner R C, Pearson M N
Department of Botany, University of Auckland, New Zealand.
Arch Virol. 1993;129(1-4):93-103. doi: 10.1007/BF01316887.
Vanilla necrosis potyvirus (VNV) is the cause of significant losses to the South Pacific islands vanilla crop. The gene for the coat protein of VNV has been cloned and sequenced. Comparison of this gene with other potyviral coat protein sequences revealed 97% nucleotide sequence homology (98% amino acid homology) to a US isolate of watermelon mosaic virus II (WMV-II), 93% nucleotide sequence homology (96% amino acid homology) to an Australian isolate of WMV-II and 81% nucleotide sequence homology (88% amino acid homology) to soybean mosaic virus-N (SMV-N). Serological analysis, by Western blot and ELISA, confirmed the close relationship between VNV and WMV-II. Furthermore, a limited host range determination found VNV and WMV-II able to infect the same series of test plants. However, symptoms differed significantly on three test species demonstrating that VNV and WMV-II are not identical in biological properties. We suggest that VNV be renamed WMV-II (Tonga).
香草坏死马铃薯Y病毒（VNV）是导致南太平洋诸岛香草作物严重损失的原因。VNV外壳蛋白基因已被克隆和测序。将该基因与其他马铃薯Y病毒外壳蛋白序列进行比较，发现其与美国分离的西瓜花叶病毒II（WMV-II）的核苷酸序列同源性为97%（氨基酸同源性为98%），与澳大利亚分离的WMV-II的核苷酸序列同源性为93%（氨基酸同源性为96%），与大豆花叶病毒-N（SMV-N）的核苷酸序列同源性为81%（氨基酸同源性为88%）。通过蛋白质免疫印迹法和酶联免疫吸附测定法进行的血清学分析证实了VNV与WMV-II之间的密切关系。此外，有限的寄主范围测定发现VNV和WMV-II能够感染同一组试验植物。然而，在三种试验物种上症状有显著差异，这表明VNV和WMV-II在生物学特性上并不相同。我们建议将VNV重新命名为WMV-II（汤加）。
