School of Life Sciences, Northeast Normal University, 5268 Renmin Street, 130024 Changchun, Jilin Province, People's Republic of China.
Med Oncol. 2011 Mar;28(1):175-81. doi: 10.1007/s12032-010-9449-8. Epub 2010 Feb 18.
Ginseng polysaccharide has anticancer activity. However, the structure-activity relationship and the activity mechanism are still unclear. Therefore, it is necessary to study the anticancer activity of structurally different ginseng polysaccharide fractions and their potential mechanisms. Ginseng polysaccharide fractions and their temperature-modified products were assayed for their effects on HT-29 cell proliferation by MTT assay, on cell cycle progression by flow cytometry, and on caspase-3 activation by western blot analysis. The HG-rich ginseng pectin inhibited cell proliferation and induced cell cycle arrest in the G2/M phase. The temperature-modified HG-rich pectin had dramatically increased antiproliferative effect and induced apoptosis accompanied by the activation of caspase-3. Starch-like glucan and arabinogalactan of ginseng exhibited no antiproliferative effects. Even after temperature modification, their inhibitory effects either remained unchanged or increased slightly. The HG-rich pectin exerts its antiproliferative effect via cell cycle arrest and the temperature modification markedly increased the antiproliferative effect.
人参多糖具有抗癌活性。然而，其构效关系和作用机制尚不清楚。因此，有必要研究结构不同的人参多糖级分的抗癌活性及其潜在机制。通过 MTT 法测定人参多糖级分及其温度修饰产物对 HT-29 细胞增殖的影响，通过流式细胞术测定细胞周期进程，通过 Western blot 分析测定半胱氨酸天冬氨酸蛋白酶-3 的激活。富含 HG 的人参果胶抑制细胞增殖并诱导细胞周期停滞在 G2/M 期。富含 HG 的经温度修饰的果胶具有显著增强的抗增殖作用，并诱导伴随半胱氨酸天冬氨酸蛋白酶-3 激活的细胞凋亡。人参淀粉样葡聚糖和阿拉伯半乳聚糖没有表现出抗增殖作用。即使经过温度修饰，它们的抑制作用要么保持不变，要么略有增加。富含 HG 的果胶通过细胞周期阻滞发挥其抗增殖作用，而温度修饰显著增强了其抗增殖作用。
