Department of Ophthalmology and Visual Sciences, Omaha, Nebraska, USA.
Stem Cells. 2010 Apr;28(4):695-703. doi: 10.1002/stem.320.
The direct reprogramming of somatic cells to a pluripotent state holds significant implications for treating intractable degenerative diseases by ex vivo cell therapy. In addition, the reprogrammed cells can serve as a model for diseases and the discovery of drugs and genes. Here, we demonstrate that mouse fibroblast induced pluripotent stem cells (iPSCs) represent a renewable and robust source of retinal progenitors, capable of generating a wide range of retinal cell types that includes retinal ganglion cells (RGCs), cone, and rod photoreceptors. They respond to simulated microenvironment of early and late retinal histogenesis by differentiating into stage-specific retinal cell types through the recruitment of normal mechanisms. The depth of the retinal potential of iPSCs suggests that they may be used to formulate stem cell approaches to understand and treat a wide range of retinal degenerative diseases from glaucoma to age-related macular degeneration (AMD).
体细胞直接重编程为多能状态，通过体外细胞治疗对治疗难治性退行性疾病具有重要意义。此外，重编程细胞可用作疾病模型以及药物和基因的发现。在这里，我们证明了小鼠成纤维细胞诱导多能干细胞（iPSCs）是视网膜祖细胞的可再生和强大来源，能够产生广泛的视网膜细胞类型，包括视网膜神经节细胞（RGC）、视锥和视杆光感受器。它们通过募集正常机制，对早期和晚期视网膜发生的模拟微环境作出反应，分化为具有特定阶段的视网膜细胞类型。iPSCs 的视网膜潜能深度表明，它们可能被用于制定干细胞方法，以理解和治疗从青光眼到年龄相关性黄斑变性（AMD）等广泛的视网膜退行性疾病。
