Sanagi M Marsin, Ling Susie L, Nasir Zalilah, Hermawan Dadan, Ibrahim Wan Aini Wan, Abu Naim Ahmedy
Universiti Teknologi Malaysia, Department of Chemistry, Faculty of Science, 81310 UTM Skudai, Johor, Malaysia.
J AOAC Int. 2009 Nov-Dec;92(6):1833-8.
LOD and LOQ are two important performance characteristics in method validation. This work compares three methods based on the International Conference on Harmonization and EURACHEM guidelines, namely, signal-to-noise, blank determination, and linear regression, to estimate the LOD and LOQ for volatile organic compounds (VOCs) by experimental methodology using GC. Five VOCs, toluene, ethylbenzene, isopropylbenzene, n-propylbenzene, and styrene, were chosen for the experimental study. The results indicated that the estimated LODs and LOQs were not equivalent and could vary by a factor of 5 to 6 for the different methods. It is, therefore, essential to have a clearly described procedure for estimating the LOD and LOQ during method validation to allow interlaboratory comparisons.
检测限（LOD）和定量限（LOQ）是方法验证中的两个重要性能特征。本研究基于国际协调会议（ICH）和欧洲化学与分子科学学会（EURACHEM）指南，比较了三种方法，即信噪比法、空白测定法和线性回归法，通过气相色谱（GC）实验方法来估算挥发性有机化合物（VOCs）的检测限和定量限。选择了甲苯、乙苯、异丙苯、正丙苯和苯乙烯这五种VOCs进行实验研究。结果表明，不同方法估算出的检测限和定量限并不等效，可能相差5至6倍。因此，在方法验证过程中，必须有一个清晰描述的估算检测限和定量限的程序，以便进行实验室间的比较。
