Lauer Wendy F, Martinez Frederic L
Bio-Rad Laboratories, 2000 Alfred Nobel Dr, Hercules, CA 94547, USA.
J AOAC Int. 2009 Nov-Dec;92(6):1871-5.
RAPID'Salmonella is a chromogenic medium for isolation and detection of Salmonella spp. in food, based on two enzymatic activities. All presumptive Salmonella-positive colonies are magenta, including lactose-positive Salmonella. S. Typhi, and S. Paratyphi serotypes, due to detection of C8 esterase activity. In order to differentiate Salmonella from other Enterobacteriaceae, the medium includes a second chromogenic substrate. As part of an Emergency Response Validation due to a massive outbreak and subsequent recall, peanut butter was tested to compare the performance of RAPID'Salmonella to the U.S. Food and Drug Administration's Bacteriological Analytical Manual reference method for detection of Salmonella. Sensitivity and specificity for RAPID'Salmonella were 100%.
RAPID沙门氏菌显色培养基是一种基于两种酶活性用于分离和检测食品中沙门氏菌属的显色培养基。所有疑似沙门氏菌阳性菌落均为品红色，包括乳糖阳性沙门氏菌、伤寒沙门氏菌和副伤寒沙门氏菌血清型，这是由于检测到C8酯酶活性。为了将沙门氏菌与其他肠杆菌科细菌区分开来，该培养基包含第二种显色底物。作为因大规模疫情爆发及随后召回而进行的应急响应验证的一部分，对花生酱进行了检测，以比较RAPID沙门氏菌显色培养基与美国食品药品监督管理局《细菌学分析手册》中检测沙门氏菌的参考方法的性能。RAPID沙门氏菌显色培养基的灵敏度和特异性均为100%。
