Shirazi Mehdi, Noorafshan Ali, Karbalay-Doust Saied, Ardeshiri Mohammad, Afrasiabi Mohammad Amin, Monabati Ahmad
Surgery Department, Division of Urology, Shahid Faghihi Hospital, Iran.
Scand J Urol Nephrol. 2010 Apr;44(3):133-7. doi: 10.3109/00365591003636588.
Spermatic cord torsion is a true urological emergency. Significant subfertility exists in patients following unilateral testicular torsion. Several studies have tested several agents such as vasodilators and antioxidants to inhibit or temper testicular damage.
Sprague-Dawley rats were divided into five groups; in group 1 (control) no operation or drug injection was done. In groups 2-5 the left testis was subjected to 270 torsions. Two hours after induced torsion, drugs were injected intraperitoneally. Rats in group 2 received no drug, whereas those in groups 3, 4 and 5 were administered a single dose of papaverine (35 mg/kg), lidocaine (4 mg/kg) and verapamil (0.1 mg/kg), respectively. Thirty minutes after drug injection (2.5 h after torsion), testis detorsion was performed in all groups. The drug injections continued for 1 week. After 20 days, bilateral orchiectomy was carried out and semen from both testes was evaluated.
In animals treated with verapamil, left testis sperm count and the percentage of motile sperm were increased significantly in comparison with the sham-operated animals. Sperm morphology in all groups was in the normal range. Mean sperm count and motility in the right testes were not significantly different in different groups.
Verapamil can induce beneficial effects on semen analysis parameters after testicular torsion in rats.
精索扭转是一种真正的泌尿外科急症。单侧睾丸扭转后的患者存在明显的生育力低下。多项研究测试了多种药物，如血管扩张剂和抗氧化剂，以抑制或减轻睾丸损伤。
将Sprague-Dawley大鼠分为五组；第1组（对照组）不进行手术或药物注射。第2 - 5组的左侧睾丸进行270度扭转。扭转诱导后两小时，腹腔内注射药物。第2组大鼠不接受药物治疗，而第3、4和5组大鼠分别给予单剂量的罂粟碱（35毫克/千克）、利多卡因（4毫克/千克）和维拉帕米（0.1毫克/千克）。药物注射30分钟后（扭转后2.5小时），所有组均进行睾丸复位。药物注射持续1周。20天后，进行双侧睾丸切除术，并对双侧睾丸的精液进行评估。
与假手术动物相比，接受维拉帕米治疗的动物左侧睾丸精子计数和活动精子百分比显著增加。所有组的精子形态均在正常范围内。不同组右侧睾丸的平均精子计数和活力无显著差异。
维拉帕米对大鼠睾丸扭转后的精液分析参数可产生有益影响。
