Prillaman H M, Turner T T
Department of Urology, University of Virginia School of Medicine, Charlottesville 22908, USA.
J Urol. 1997 Jan;157(1):340-5.
Spermatic cord torsion results in impairment of testicular function. The mechanism of this injury is unclear; however, intracellular Ca++ influx and reactive oxygen species have been implicated in the testicular damage following torsion and reperfusion. In the present study a model of testicular torsion in the rat was used to determine whether testicular function following torsion can be rescued by the administration of antioxidants and Ca++ channel blockers.
Seventy-two animals were divided into 9 groups. Animals underwent 1 hr. or 2 hrs., 720 degrees experimental torsion. Animals received combinations of superoxide dismutase (SOD), catalase, allopurinol, and verapamil. Drugs were administered intravenously during the last 15 minutes of experimental torsion and the first hour of reperfusion. Bilateral testicular function was determined 60 days after experimental torsion by measuring testis weights, daily sperm production (DSP), and testicular venous testosterone concentrations. Ipsilateral values were compared to both control and contralateral values.
SOD + catalase and SOD + catalase+verapamil treatments caused significant rescue of tests function following 1 hr. experimental torsion. Mean +/- s.e. testis weights and DSP in control animals were 1.75 +/- 0.6 g. and 18.4 +/- 0.3 x 10(6) sperm/g./d. The same value for testes experiencing 1 hr. experimental torsion were 0.72 +/- 0.6 g. and 2.3 +/- 0.5 x 10(6) sperm/g./d. The values from testes receiving 1 hr. experimental torsion followed by SOD + catalase were 1.45 +/- 0.17 g. and 9.9 +/- 1.8 x 10(6) sperm/g./d. Neither allopurinol nor verapamil added benefit. No significant rescue was seen in testes undergoing 2 hrs. experimental torsion.
Treatment with oxygen radical scavengers provides significant rescue of testicular function after acute experimental torsion.
精索扭转会导致睾丸功能受损。这种损伤的机制尚不清楚；然而，细胞内钙离子内流和活性氧已被认为与扭转和再灌注后的睾丸损伤有关。在本研究中，使用大鼠睾丸扭转模型来确定给予抗氧化剂和钙离子通道阻滞剂是否能挽救扭转后的睾丸功能。
72只动物被分为9组。动物经历1小时或2小时、720度的实验性扭转。动物接受超氧化物歧化酶（SOD）、过氧化氢酶、别嘌呤醇和维拉帕米的联合使用。在实验性扭转的最后15分钟和再灌注的第一小时静脉给药。实验性扭转60天后，通过测量睾丸重量、每日精子生成量（DSP）和睾丸静脉睾酮浓度来确定双侧睾丸功能。将同侧值与对照值和对侧值进行比较。
SOD + 过氧化氢酶和SOD + 过氧化氢酶 + 维拉帕米治疗在1小时实验性扭转后能显著挽救睾丸功能。对照动物的平均±标准误睾丸重量和DSP分别为1.75±0.6克和18.4±0.3×10⁶精子/克/天。经历1小时实验性扭转的睾丸的相同值为0.72±0.6克和2.3±0.5×10⁶精子/克/天。接受1小时实验性扭转后给予SOD + 过氧化氢酶的睾丸的值为1.45±0.17克和9.9±1.8×10⁶精子/克/天。别嘌呤醇和维拉帕米均未增加益处。在经历2小时实验性扭转的睾丸中未观察到显著的挽救作用。
用氧自由基清除剂治疗可在急性实验性扭转后显著挽救睾丸功能。
