Bloom G D, Carlsöö B, Henriksson R
J Laryngol Otol. 1977 Nov;91(11):947-958. doi: 10.1017/s0022215100084619.
The ultrastructure of an acinic cell carcinoma, occurring in the left parotid gland of a 52-year-old woman and causing a total facial nerve paralysis, is described. Histologically the tumour consisted of numerous granulated cells arranged around lumen-like openings and resembling a secretory system. Furthermore, areas with agranulated cells growing in a solid pattern were also encountered. In the electron microscope the cytoplasmic granules of the tumour cells displayed a varied appearance. Granules of a dense homogeneous type, as well as granules with a more electron lucid appearance were observed. Furthermore, numerous cytoplasmic granules displayed a bipartite structure with a dense central and a more electron lucid outer zone. In specimens primarily fixed in OSO4 or KMnO4 the granules displayed a 'leached out' appearance. The membrane-bounded of the tumour cells also showed a strong positive staining with the periodic acid-chromic silver technique of Rambourg et al. (1969). Other characteristic ultrastructural features of the tumour cells studied were: Smooth cell surfaces, the presence of subplasmalemmal bands of electron dense material, desmosome-like attachment areas between cells and grossly altered mitochondria.
描述了发生在一名52岁女性左腮腺的腺泡细胞癌的超微结构，该肿瘤导致了完全性面神经麻痹。组织学上，肿瘤由围绕管腔样开口排列且类似分泌系统的大量颗粒细胞组成。此外，还发现有呈实性生长的无颗粒细胞区域。在电子显微镜下，肿瘤细胞的胞质颗粒呈现出多样的外观。观察到致密均质型颗粒以及外观更具电子透明性的颗粒。此外，许多胞质颗粒呈现出二分结构，有致密的中央区和更具电子透明性的外周区。在主要用四氧化锇（OSO4）或高锰酸钾固定的标本中，颗粒呈现出“洗脱”的外观。肿瘤细胞的膜结合物在用Rambourg等人（1969年）的高碘酸 - 铬银技术染色时也显示出强阳性。所研究的肿瘤细胞的其他特征性超微结构特点包括：细胞表面光滑、存在电子致密物质的质膜下带、细胞间类似桥粒的附着区域以及明显改变的线粒体。
