Bio-Nano Electronics Research Centre, Toyo University, Saitama 350-8585, Japan.
Biochem Biophys Res Commun. 2010 Mar 19;393(4):779-82. doi: 10.1016/j.bbrc.2010.02.081. Epub 2010 Feb 18.
We immobilize alpha-amylase extracted from Bacillus Iicheniformis on the surfaces of superparamagnetic particles and investigate the effect of a rotational magnetic field on the enzyme's activity. We find that the activity of the enzyme molecules immobilized on superparamagnetic particles increases in the rotational magnetic field and reaches maximum at a certain frequency. We clarify the effect of the cluster structures formed by the superparamagnetic particles on the activity. Enzyme reactions are enhanced even in a tiny volume of solution using the present method, which is very important for the development of efficient micro reactors and micro total analysis systems (mu-TAS).
我们将地衣芽孢杆菌中提取的α-淀粉酶固定在超顺磁性颗粒的表面上，并研究了旋转磁场对酶活性的影响。我们发现，固定在超顺磁性颗粒上的酶分子的活性在旋转磁场中增加，并在一定频率下达到最大值。我们阐明了超顺磁性颗粒形成的簇结构对活性的影响。使用本方法即使在微小体积的溶液中也能增强酶反应，这对于高效微反应器和微全分析系统（μ-TAS）的发展非常重要。
