Izuhara M, Takamune K, Takata R
Department of Biology, Saga Medical School, Nabeshima, Japan.
Mol Gen Genet. 1991 Mar;225(3):510-3. doi: 10.1007/BF00261694.
Using lambda phage clones containing segments of the Escherichia coli K12 chromosome as hybridization probes, we found one gene at 42 min on the E. coli chromosome map, the expression of which was affected by RNase III. The sequence of the DNA fragment containing this gene (gen-165) revealed the presence of an open reading frame encoding a polypeptide of 165 amino acid residues. The amino acid sequence deduced from the nucleotide sequence exhibited a remarkable similarity to that of the human ferritin H chain.
使用含有大肠杆菌K12染色体片段的λ噬菌体克隆作为杂交探针，我们在大肠杆菌染色体图谱上42分钟处发现了一个基因，其表达受核糖核酸酶III影响。包含该基因（gen-165）的DNA片段序列显示存在一个编码165个氨基酸残基多肽的开放阅读框。从核苷酸序列推导的氨基酸序列与人类铁蛋白H链的氨基酸序列具有显著相似性。
