Department of Anatomy & Neurobiology, Hainan Medical College, Haikou 571101, Hainan, China.
J Chem Neuroanat. 2010 Jul;39(4):256-61. doi: 10.1016/j.jchemneu.2010.02.001. Epub 2010 Feb 19.
Secreted Slit proteins have previously been shown to signal through Roundabout (Robo) receptors to negatively regulate axon guidance and cell migration. During vertebrate development, Slit proteins have also been shown to stimulate branching and elongation of sensory axons and cortical dendrites. In this study, Slit1/Robo2 mRNA and protein expressions were detected in adult rat dorsal root ganglion (DRG) and in cultured DRG neurons. Treatment of both models with recombinant, soluble Slit1 protein was found to promote neurite outgrowth and elongation. In contrast, treatment with a recombinant human Robo2/Fc chimera inhibited neurite outgrowth and elongation. When adult DRG and cultured DRG neurons were pretreated with soluble recombinant human Robo2/Fc chimera, neurite outgrowth and elongation was not induced. These findings indicate that Slit1/Robo2 signaling may have a role in regulating peripheral nerve regeneration.
分泌型 Slit 蛋白先前已被证明通过 Roundabout（Robo）受体发出信号，从而负调控轴突导向和细胞迁移。在脊椎动物发育过程中，Slit 蛋白还被证明可刺激感觉轴突和皮质树突的分支和伸长。在这项研究中，检测到成年大鼠背根神经节（DRG）和培养的 DRG 神经元中 Slit1/Robo2 mRNA 和蛋白的表达。用重组可溶性 Slit1 蛋白处理这两种模型均发现可促进神经突的生长和伸长。相比之下，用重组人 Robo2/Fc 嵌合体处理可抑制神经突的生长和伸长。当用可溶性重组人 Robo2/Fc 嵌合体预处理成年 DRG 和培养的 DRG 神经元时，不会诱导神经突的生长和伸长。这些发现表明 Slit1/Robo2 信号可能在调节周围神经再生中发挥作用。
