Nutritional Intervention Research Unit, Medical Research Council, Tygerberg 7505, Cape Town, South Africa.
Best Pract Res Clin Endocrinol Metab. 2010 Feb;24(1):77-88. doi: 10.1016/j.beem.2009.08.006.
Good quality data on iodine concentrations in urine and salt samples are indispensable for the efficient management of national salt iodisation programmes and for evaluating iodine interventions. Most of the analytical methods for urinary iodine concentration are based on the manual spectrophotometric measurement of Sandell-Kolthoff reduction reaction catalysed by iodine using different oxidising reagents in the initial digestion step. Other analytical methods include semi-quantitative methods, a microplate method, automated methods; and the technologically advanced methods include the inductively coupled plasma mass-spectrometer method. Iodine in salt is determined quantitatively by the titration method, colorimetrically by the WYD iodine checker or by a technologically advanced potentiometric method. Worldwide, titration is the method of choice because of its accuracy, ease of operation and low cost. Rapid test kits are suitable for qualitative use in situations where iodised salt need to be distinguished from non-iodised salt, preferably with titration back-up.
高质量的尿碘和盐碘样本数据对于有效管理国家盐碘规划和评估碘干预措施是必不可少的。大多数尿碘浓度的分析方法都是基于手工分光光度法，利用不同的氧化剂在初始消化步骤中催化碘的 Sandell-Kolthoff 还原反应。其他分析方法包括半定量方法、微孔板方法、自动化方法；以及技术先进的方法，包括电感耦合等离子体质谱法。盐中的碘通过滴定法定量测定，通过 WYD 碘检测仪比色法或技术先进的电位法测定。在全球范围内，由于其准确性、易于操作和低成本，滴定法是首选方法。快速检测试剂盒适用于定性使用，用于区分碘盐和非碘盐，最好有滴定法作为后盾。
