Fuentes V, Anarratone F, Froissart M, Orfila J
Laboratoire de Bactériologie-Immunologie Générale, CHRU, Amiens, France.
Pathol Biol (Paris). 1991 Feb;39(2):117-21.
This study was undertaken to evaluate the diagnostic value of detection and titration of specific IgA antibodies against Chlamydia trachomatis in active infections of the upper genital tract. Three different techniques using L2 serovar antigens were compared: immunofluorescence on cells infected with Chlamydia trachomatis, microimmunofluorescence as described by Wang and Grauston, and an indirect immunoperoxidase method on infected cells. Good correlations were found between the three techniques. Results demonstrated that the cutoff level should be 1/16.
本研究旨在评估检测和滴定抗沙眼衣原体特异性IgA抗体在上生殖道活动性感染中的诊断价值。比较了三种使用L2血清型抗原的不同技术：对感染沙眼衣原体的细胞进行免疫荧光检测、采用Wang和Grauston描述的微量免疫荧光检测法以及对感染细胞进行间接免疫过氧化物酶法。发现这三种技术之间具有良好的相关性。结果表明临界值应为1/16。
