Welsh J, McClelland M
California Institute of Biological Research, La Jolla 92037.
Mol Microbiol. 1992 Jun;6(12):1673-80. doi: 10.1111/j.1365-2958.1992.tb00892.x.
The intergenic spacers between some adjacent tRNA genes were shown to be polymorphic in length when closely related Staphylococcus species were compared. A simple procedure was developed to detect and sequence these tRNA intergenic length polymorphisms (tRNA-ILPs). A comparison of homologous tRNA gene sequences flanking these ILPs in three Staphylococcus species was used to derive primers for high-stringency amplification of the ILPs by the polymerase chain reaction (PCR). The detection of tRNA-ILPs by PCR allowed the classification of virtually all strains from the five species of Staphylococcus that were examined. The procedure used to identify, sequence and derive primers for PCR detection of tRNA-ILPs in Staphylococcus should be applicable to many other genera of eubacteria. These primers could be used on uncultured material such as clinical samples.
当比较亲缘关系较近的葡萄球菌属物种时，发现一些相邻tRNA基因之间的基因间隔区长度具有多态性。开发了一种简单的程序来检测这些tRNA基因间隔区长度多态性（tRNA-ILP）并进行测序。通过比较三种葡萄球菌属物种中这些ILP侧翼的同源tRNA基因序列，设计引物用于通过聚合酶链反应（PCR）对ILP进行高严格度扩增。通过PCR检测tRNA-ILP可以对所检测的葡萄球菌属五个物种的几乎所有菌株进行分类。用于鉴定、测序以及设计PCR检测葡萄球菌属tRNA-ILP引物的程序应该适用于许多其他真细菌属。这些引物可用于未培养的材料，如临床样本。
