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四种伯氏疏螺旋体菌株的限制性内切酶分析。

Restriction endonuclease analysis of four Borrelia burgdorferi strains.

作者信息

Cinco M, Banfi E, Balanzin D, Cacciò S, Graziosi G, Fattorini P

机构信息

Istituto di Microbiologia, Università di Trieste, Italy.

出版信息

FEMS Microbiol Immunol. 1989 Dec;1(8-9):511-4. doi: 10.1111/j.1574-6968.1989.tb02443.x.

DOI:10.1111/j.1574-6968.1989.tb02443.x
PMID:2560664
Abstract

A restriction endonuclease analysis was performed on four strains of Borrelia burgdorferi: one isolated from man (SF), one from Ixodes dammini (B31) and two from I. ricinus (BITS in Italy and B45 in Germany). Digestion by Taq I and Hae III gave the best resolution of the DNA fragments. Three different restriction patterns were obtained: BITS and B45 showed only one band difference. These results correlate with the reactivity of the four strains with monoclonal antibodies.

摘要

对四株伯氏疏螺旋体进行了限制性内切酶分析

一株从人身上分离得到(SF),一株从达米尼硬蜱(B31)分离得到,两株从蓖麻硬蜱分离得到(意大利的BITS和德国的B45)。用Taq I和Hae III进行消化能使DNA片段得到最佳分辨率。获得了三种不同的限制性图谱:BITS和B45仅显示出一条带的差异。这些结果与这四株菌株与单克隆抗体的反应性相关。

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引用本文的文献

1
Protein and antigenic analysis of Borrelia burgdorferi isolated in northern Italy: computerized analysis of phenotypic characteristics.意大利北部分离出的伯氏疏螺旋体的蛋白质和抗原分析:表型特征的计算机分析
J Clin Microbiol. 1993 Feb;31(2):440-3. doi: 10.1128/jcm.31.2.440-443.1993.
2
Use of polymerase chain reaction and specific monoclonal antibodies as rapid method to recognize Borrelia burgdorferi sensu stricto, B. garinii and B. afzelii among Italian isolates of B. burgdorferi.使用聚合酶链反应和特异性单克隆抗体作为快速方法,以识别意大利疏螺旋体分离株中的狭义伯氏疏螺旋体、伽氏疏螺旋体和阿氏疏螺旋体。
Med Microbiol Immunol. 1994 Dec;183(6):307-13. doi: 10.1007/BF00196681.
3
Genomic fingerprints produced by PCR with consensus tRNA gene primers.
使用共有tRNA基因引物通过聚合酶链反应产生的基因组指纹图谱。
Nucleic Acids Res. 1991 Feb 25;19(4):861-6. doi: 10.1093/nar/19.4.861.