Cinco M, Banfi E, Balanzin D, Cacciò S, Graziosi G, Fattorini P
Istituto di Microbiologia, Università di Trieste, Italy.
FEMS Microbiol Immunol. 1989 Dec;1(8-9):511-4. doi: 10.1111/j.1574-6968.1989.tb02443.x.
A restriction endonuclease analysis was performed on four strains of Borrelia burgdorferi: one isolated from man (SF), one from Ixodes dammini (B31) and two from I. ricinus (BITS in Italy and B45 in Germany). Digestion by Taq I and Hae III gave the best resolution of the DNA fragments. Three different restriction patterns were obtained: BITS and B45 showed only one band difference. These results correlate with the reactivity of the four strains with monoclonal antibodies.
一株从人身上分离得到(SF),一株从达米尼硬蜱(B31)分离得到,两株从蓖麻硬蜱分离得到(意大利的BITS和德国的B45)。用Taq I和Hae III进行消化能使DNA片段得到最佳分辨率。获得了三种不同的限制性图谱:BITS和B45仅显示出一条带的差异。这些结果与这四株菌株与单克隆抗体的反应性相关。