Bishop K D, Borer P N, Huang Y Q, Lane M J
Department of Chemistry, Syracuse University, NY 13244-4100.
Nucleic Acids Res. 1991 Feb 25;19(4):871-5. doi: 10.1093/nar/19.4.871.
DNase I cleavage rates and nmr chemical shifts are shown to change for DNA sequences distal to an intercalated actinomycin D molecule in a duplex hexadecamer upon drug binding. Both sets of observations suggest that the source of these changes is a DNA-mediated structural response. The nmr results imply the response is transmitted preferentially in a 5'-to-3' direction from the drug binding site. An inequivalent response of the two strands to a ligand-induced conformational change immediately suggests a mechanism for distinguishing the sense and antisense strands of DNA.
在双链十六聚体中，当放线菌素D分子嵌入后，与嵌入药物远端的DNA序列相关的DNA酶I切割速率和核磁共振化学位移会发生变化。这两组观察结果均表明，这些变化源于DNA介导的结构响应。核磁共振结果表明，这种响应优先从药物结合位点沿5'至3'方向传递。两条链对配体诱导的构象变化的不等价响应立即提示了一种区分DNA有义链和反义链的机制。
