Department of Biomedical Engineering, Center on Biomaterials, Drug Delivery, Bionanotechnology and Molecular Recognition, The University of Texas at Austin, 1 University Station, C0400, Austin, TX 78712-0231, USA.
Biomed Microdevices. 2010 Jun;12(3):435-42. doi: 10.1007/s10544-010-9400-y.
Human disease processes are often characterized by a deviation from the normal physiological concentration of critical biomarkers. The detection of disease biomarkers requires the development of novel sensing methods which are sensitive, specific, efficient and low-cost. To address this need, the ability of a device, which incorporates a film of polymer acid doped polyaniline, to respond to proteins at physiological pH and ionic strength was assessed. The conductive polymer was found to respond by changing conductivity in the presence of biomolecules, demonstrating a direct chemical to electronic transduction method. In future work, specificity can be incorporated into the system by integrating the conductive polymer with a protein selective film. The demonstration of a conductive polymer which is responsive to proteins at physiological conditions is a step towards the integration of these materials into implantable sensing systems.
人体疾病过程通常表现为关键生物标志物的正常生理浓度发生偏差。疾病生物标志物的检测需要开发新型传感方法，这些方法应具有灵敏度高、特异性强、效率高和成本低的特点。为满足这一需求，评估了一种设备的能力，该设备包含掺杂有聚合物酸的聚苯胺薄膜，可以在生理 pH 值和离子强度下响应蛋白质。研究发现，导电聚合物在存在生物分子的情况下通过改变电导率来响应，证明了一种直接的化学到电子转换方法。在未来的工作中，可以通过将导电聚合物与蛋白质选择性薄膜集成来将特异性纳入系统。在生理条件下对蛋白质有响应的导电聚合物的演示是将这些材料集成到可植入传感系统的重要一步。
