Graduate School of Semiconductor and Chemical Engineering, Chonbuk National University, 664-14 Duckjin-dong, Jeonju 561-756, South Korea.
Mol Cell Biochem. 2010 Jul;340(1-2):175-8. doi: 10.1007/s11010-010-0415-8. Epub 2010 Feb 20.
Lysosomes are a cell organelle type processing antimicrobial activity. Here, we investigate the lysosomal activity in a primary cell line, bovine aortic endothelial cells (BAECs), during cellular aging, based on the antimicrobial activity of lysosomes isolated from BAECs at cell passages 4, 6, 8, and 10. Cytochemical analysis of lysosomes with LysoTracker reagent revealed the number of lysosome-like organelles surrounding the nucleus initially increased drastically in the BAECs and continued increasing gradually until passage 10. The lysosomes isolated from each successive passage of BAECs exhibited increased antimicrobial activity against Escherichia coli, and, in addition, an age-dependent increase in lysosome intensity coincided with increased lysosomal antimicrobial activity.
溶酶体是一种具有抗菌活性的细胞细胞器。在这里，我们基于从细胞传代 4、6、8 和 10 的牛主动脉内皮细胞 (BAEC) 中分离的溶酶体的抗菌活性，研究了 BAEC 细胞衰老过程中的溶酶体活性。用 LysoTracker 试剂对溶酶体进行细胞化学分析显示，细胞核周围的溶酶体样细胞器数量最初急剧增加，在 BAEC 中持续增加，直到传代 10。从 BAEC 的每个连续传代中分离出的溶酶体对大肠杆菌的抗菌活性增加，此外，溶酶体强度与抗菌活性呈年龄依赖性增加。
