两株担子菌 Cerrena unicolor VKMF-3196 的漆酶同工酶。诱导、分离与性质。
Two laccase isoforms of the basidiomycete Cerrena unicolor VKMF-3196. Induction, isolation and properties.
机构信息
G.K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences, Russian Federation, Pushchino, Moscow region, Prospekt Nauki.
出版信息
J Basic Microbiol. 2010 Feb;50(1):72-82. doi: 10.1002/jobm.200900382.
The laccase induction in submerged culture of basidiomycete Cerrena unicolor VKM F-3196 was investigated. Cu(2+) at concentration 0.1 mM was an optimum inducer of C. unicolor laccase. Two isoforms of laccase, namely LacC1 and LacC2, were isolated and characterized. The isoforms were shown to have different physical-chemical and catalytic properties. On the basis of the MALDI TOF MS analysis of tryptic cleavage products of both the proteins and N-terminal amino-acid sequences analysis two isoforms of laccase (LacC1 and LacC2) were classified as products of two different genes.
研究了担子菌 Cerrena unicolor VKM F-3196 液体深层培养中的漆酶诱导。Cu(2+)浓度为 0.1mM 时是 C. unicolor 漆酶的最佳诱导剂。分离并表征了两种同工酶，即 LacC1 和 LacC2。结果表明，这两种同工酶具有不同的理化性质和催化特性。基于两种蛋白质的胰蛋白酶裂解产物的 MALDI TOF MS 分析和 N 末端氨基酸序列分析，将两种同工酶（LacC1 和 LacC2）归类为两个不同基因的产物。
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