Department of Psychobiology-Universidade Federal de São Paulo, Escola Paulista de Medicina (UNIFESP/EPM), Brazil.
Sleep. 2010 Feb;33(2):243-51. doi: 10.1093/sleep/33.2.243.
To assess the influence of total or selective REM sleep deprivation on the dopamine transporter (DAT) densities and sleep patterns of healthy volunteers.
Prospective study.
Evaluation of polysomnography recordings and DAT density after 4 nights of selective REM sleep deprivation followed by 3 nights of sleep recovery compared to a control group and a group that was subjected to 2 nights of total sleep deprivation. Single positron emission computed tomography and [99mTc]TRODAT-1 were used to assess the cerebral DAT density in the striatum at baseline, after REM sleep deprivation and total sleep deprivation as well as after sleep recovery. Blood was collected daily to examine prolactin and estradiol levels, which were correlated with dopaminergic activity.
Thirty healthy male volunteers ranging from 19 to 29 years of age were randomly assigned to one of three experimental groups after giving written informed consent (10 non-sleep deprived, 10 total sleep deprived, and 10 REM sleep deprived).
Four nights of REM sleep deprivation and 2 nights of total sleep deprivation induced distinct and heterogeneous patterns of sleep recovery. No significant modulation of DAT availability was observed within groups. In the recovery nights, changes in cortisol, prolactin and estradiol concentrations were significantly correlated with specific sleep stages in the total and REM sleep deprived groups. In addition, DAT density was positively correlated with estradiol concentration and inversely associated with SWS latency only after total sleep deprivation.
Our study demonstrates that although sleep deprivation did not promote significant alterations in DAT density within the striatum, there were significant correlations among transporter availability, hormonal concentrations and sleep parameters.
评估完全或选择性 REM 睡眠剥夺对健康志愿者的多巴胺转运体 (DAT) 密度和睡眠模式的影响。
前瞻性研究。
评估多导睡眠图记录和 4 晚选择性 REM 睡眠剥夺后 3 晚睡眠恢复后的 DAT 密度，与对照组和 2 晚总睡眠剥夺组进行比较。单光子发射计算机断层扫描和 [99mTc]TRODAT-1 用于评估基线、REM 睡眠剥夺和总睡眠剥夺以及睡眠恢复后纹状体中的大脑 DAT 密度。每天采集血液以检查催乳素和雌二醇水平，这些水平与多巴胺能活性相关。
30 名年龄在 19 至 29 岁之间的健康男性志愿者在书面知情同意后被随机分配到三个实验组之一（10 名非睡眠剥夺者、10 名总睡眠剥夺者和 10 名 REM 睡眠剥夺者）。
4 晚 REM 睡眠剥夺和 2 晚总睡眠剥夺引起了不同和异质的睡眠恢复模式。组内 DAT 可用性无明显变化。在恢复夜间，皮质醇、催乳素和雌二醇浓度的变化与总睡眠剥夺和 REM 睡眠剥夺组特定睡眠阶段显著相关。此外，DAT 密度与雌二醇浓度呈正相关，仅与总睡眠剥夺后 SWS 潜伏期呈负相关。
我们的研究表明，尽管睡眠剥夺没有促进纹状体中 DAT 密度的显著变化，但转运体可用性、激素浓度和睡眠参数之间存在显著相关性。
