Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Hradec Kralove, Czech Republic.
J Proteome Res. 2010 Apr 5;9(4):1995-2005. doi: 10.1021/pr9011602.
It appears that most glycoproteins found in pathogenic bacteria are associated with virulence. Despite the recent identification of novel virulence factors, the mechanisms of virulence in Francisella tularensis are poorly understood. In spite of its importance, questions about glycosylation of proteins in this bacterium and its potential connection with bacterial virulence have not been answered yet. In the present study, several putative Francisella tularensis glycoproteins were characterized through the combination of carbohydrate-specific detection and lectin affinity with highly sensitive mass spectrometry utilizing the bottom-up proteomic approach. The protein PilA that was recently found as being possibly glycosylated, as well as other proteins with designation as novel factors of virulence, were among the proteins identified in this study. The reported data compile the list of potential glycoproteins that may serve as a takeoff platform for a further definition of proteins modified by glycans, faciliting a better understanding of the function of protein glycosylation in pathogenicity of Francisella tularensis.
似乎大多数在致病性细菌中发现的糖蛋白都与毒力有关。尽管最近发现了新的毒力因子，但弗朗西斯氏菌的毒力机制仍未得到很好的理解。尽管它很重要，但关于该细菌中蛋白质的糖基化及其与细菌毒力的潜在联系的问题尚未得到解答。在本研究中，通过结合碳水化合物特异性检测和糖基结合蛋白与利用基于 Bottom-up 的蛋白质组学方法的高灵敏度质谱联用，对几种假定的弗朗西斯氏菌糖蛋白进行了表征。最近发现可能糖基化的 PilA 蛋白以及其他被指定为新的毒力因子的蛋白均是本研究中鉴定到的蛋白之一。所报道的数据汇集了可能的糖蛋白列表，这些糖蛋白可能作为进一步定义糖基化修饰蛋白的起点，有助于更好地理解蛋白质糖基化在弗朗西斯氏菌致病性中的作用。
