马疱疹病毒1型和3型的遗传相关性。
Genetic relatedness of equine herpesvirus types 1 and 3.
作者信息
Allen G P, O'Callaghan D J, Randall C C
出版信息
J Virol. 1977 Dec;24(3):761-7. doi: 10.1128/JVI.24.3.761-767.1977.
Abstract
The genetic relatedness of two types of equine herpesviruses (EHVs), 1 (EHV-1) and 3 (EHV-3), was determined by DNA-DNA reassociation kinetics. Denatured, labeled viral DNA probe was allowed to reassociate in the presence or absence of the second unlabeled viral DNA. The initial rate of reassociation of either labeled viral DNA was increased by the presence of the heterologous viral DNA to an extent indicating only 2 to 5% homology between the two EHV genomes. Moreover, labeled RNA extracted from EHV-3-infected cells hybridized to filter-immobilized EHV-1 DNA only 2 to 3 percent as efficiently as to the homologous EHV-3 DNA. These results demonstrate that the genital (EHV-3) and nongenital (EHV-1) types of EHVs exhibit very little genetic homology.
摘要
通过DNA-DNA重缔合动力学确定了两种马疱疹病毒（EHVs），即1型（EHV-1）和3型（EHV-3）的遗传相关性。使变性的、标记的病毒DNA探针在存在或不存在第二种未标记的病毒DNA的情况下进行重缔合。任一标记病毒DNA的初始重缔合速率因异源病毒DNA的存在而增加，增加程度表明两个EHV基因组之间只有2%至5%的同源性。此外，从EHV-3感染细胞中提取的标记RNA与固定在滤膜上的EHV-1 DNA杂交的效率仅为与同源EHV-3 DNA杂交效率的2%至3%。这些结果表明，EHV的生殖道型（EHV-3）和非生殖道型（EHV-1）表现出很少的遗传同源性。
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