Micología Médica, Facultad de Medicina, Universidad Nacional Autónoma de México, Mexico City, Mexico.
Mycopathologia. 2010 Jun;169(6):427-30. doi: 10.1007/s11046-010-9280-y. Epub 2010 Feb 23.
Coccidioidin, an extract from the saprophytic mycelial form of Coccidioides spp., has been a very useful antigen preparation both for skin and serological tests for coccidioidomycosis. Unfortunately, coccidioidin is not currently available for skin testing in the United States. Coccidioidin has been produced commercially in Mexico by a vaccine and reagents laboratory of the Mexican Federal Government. It also has been produced at the Microbiology Laboratory of the Faculty of Medicine, Universidad Nacional Autónoma de México exclusively as an antigen for research projects. The objective of the study was to compare both coccidioidins in their reactivity and safety when applied in humans. One hundred and eighty-four volunteers were tested; median age was 33 (range 14-82). When the cutoff point is set in 5 mm, 88 subjects (47.8%) had a positive test for the commercial coccidioidin and 76 (41.3%; CI(95%) 0.50, 1.15; P = 0.20) were positive with the research antigen. Seventy-five subjects were positive for both antigens and 96 were negative for both. Fifty-nine subjects (31.3%) reported an adverse reaction after the application of the antigen; they were mostly very mild local reactions. Mexican research coccidioidin is a safe and reliable antigen that can be used for the detection of coccidioidomycosis infection in mammals.
粗球菌素是粗球菌属（Coccidioides spp.）腐生菌的菌丝提取物，它是一种非常有用的抗原制剂，可用于皮肤和血清学检测球孢子菌病。不幸的是，粗球菌素目前在美国尚未用于皮肤测试。粗球菌素已由墨西哥联邦政府的一家疫苗和试剂实验室在墨西哥商业化生产，也由墨西哥国立自治大学医学系微生物学实验室专门作为研究项目的抗原生产。本研究的目的是比较这两种粗球菌素在人体应用中的反应性和安全性。共有 184 名志愿者接受了测试；中位数年龄为 33 岁（范围 14-82 岁）。当截断值设定为 5 毫米时，88 名受试者（47.8%）对商业粗球菌素的检测呈阳性，76 名受试者（41.3%；95%CI 0.50，1.15；P=0.20）对研究抗原呈阳性。75 名受试者两种抗原均为阳性，96 名受试者两种抗原均为阴性。59 名受试者（31.3%）在应用抗原后出现不良反应；它们主要是非常轻微的局部反应。墨西哥研究用粗球菌素是一种安全可靠的抗原，可用于检测哺乳动物中的球孢子菌病感染。
