Toriello C, Reyes-Montes M R, Taylor M L
Departamento de Microbiología y Parasitología, Facultad de Medicina, Universidad Nacional Autónoma de México, México, D.F.
Rev Invest Clin. 1997 Nov-Dec;49(6):501-5.
Fungal antigens with good reactivity and specificity are essential for the immunodiagnosis of systemic mycoses. We give here data on crude and purified antigens of Histoplasma capsulatum, Coccidioidis immitis and Paracoccidioides brasiliensis from local strains and which are the more prevalent in Mexico. The crude antigens had good reactivity in precipitating and skin testing whereas the purified antigens (DPPC: deproteinized polysaccharide protein complex) had a higher specificity in more sensitive tests such as ELISA and complement fixation. Our efficiency analysis showed that the crude antigens are best for epidemiologic studies due to their low cost, easy handling and fast detection. The purified ones are suited to establish with more precision the diagnosis of systemic mycoses.
具有良好反应性和特异性的真菌抗原对于系统性真菌病的免疫诊断至关重要。我们在此提供关于来自本地菌株的荚膜组织胞浆菌、粗球孢子菌和巴西副球孢子菌的粗抗原和纯化抗原的数据，这些菌株在墨西哥更为常见。粗抗原在沉淀试验和皮肤试验中具有良好的反应性，而纯化抗原（DPPC：脱蛋白多糖蛋白复合物）在ELISA和补体结合等更敏感的试验中具有更高的特异性。我们的效率分析表明，粗抗原因其成本低、易于操作和检测快速，最适合用于流行病学研究。纯化抗原则更适合于更精确地确立系统性真菌病的诊断。
