Institute for Diagnosis and Animal Health, National Reference Laboratory for Rabies, Dr. Staicovici no 63, district 5, 050557 Bucharest, Romania.
Virus Res. 2010 Jun;150(1-2):28-33. doi: 10.1016/j.virusres.2010.02.008. Epub 2010 Feb 21.
Rabies has been present in Romania since antiquity and remains a continual threat to veterinary and public health. The current epizootic of rabies in Romania is fox mediated. However, little is known about the genetic diversity among rabies virus isolates from this part of the Balkan region. In this study, we analyzed a cohort of 64 rabies viruses isolated from infected animals in Romania collected between 2005 and 2008. Partial nucleoprotein sequence data (322 bp) were obtained and compared to a panel of available sequence data of Eurasian classical rabies virus (genotype 1) isolates. The Romanian sequences were identified as belonging to the cosmopolitan lineage of rabies viruses that have been reported throughout the world. The sequences showed a high degree of heterogeneity and six different lineages were identified. Single isolates clustered with the formerly established Eastern European group, the North-Eastern European group or with isolates from the Southwest of Russia. However two lineages were unique to Romania. This sequence diversity is unprecedented in European countries and could be a result of trans-border movement or remnants of previous epidemic waves.
狂犬病在罗马尼亚由来已久，一直对兽医和公共卫生构成威胁。罗马尼亚目前的狂犬病流行是由狐狸传播的。然而，人们对来自巴尔干地区这一地区的狂犬病病毒分离株的遗传多样性知之甚少。在这项研究中，我们分析了 2005 年至 2008 年间从罗马尼亚感染动物中分离出的 64 株狂犬病病毒。获得了部分核蛋白序列数据（322bp），并与一组可用的欧亚古典狂犬病病毒（基因型 1）分离株的序列数据进行了比较。罗马尼亚序列被鉴定为属于在世界范围内报告的狂犬病病毒普遍谱系。这些序列显示出高度的异质性，确定了六个不同的谱系。单一分离株与以前确定的东欧群、东北欧群或来自俄罗斯西南部的分离株聚类。然而，有两个谱系是罗马尼亚特有的。这种序列多样性在欧洲国家是前所未有的，可能是跨境传播或以前流行波的残余造成的。
