萜类化合物橙花叔醇对巴贝斯虫寄生虫生长的抑制作用。

Inhibitory effect of terpene nerolidol on the growth of Babesia parasites.

作者信息

AbouLaila Mahmoud, Sivakumar Thillaiampalam, Yokoyama Naoaki, Igarashi Ikuo

机构信息

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan.

出版信息

Parasitol Int. 2010 Jun;59(2):278-82. doi: 10.1016/j.parint.2010.02.006. Epub 2010 Feb 21.

DOI:10.1016/j.parint.2010.02.006

PMID:20178862

Abstract

Nerolidol is a sesquiterpene present in the essential oils of many plants, approved by the U.S. FDA as a food flavoring agent. Nerolidol interferes with the isoprenoid biosynthetic pathway in the apicoplast of P. falciparum. In the present study, the in vitro growth of four Babesia species was significantly (P<0.05) inhibited in the presence of nerolidol (IC(50)s values=21+/-1, 29.6+/-3, 26.9+/-2, and 23.1+/-1microM for B. bovis, B. bigemina, B. ovata, and B. caballi, respectively). Parasites from treated cultures failed to grow in the subsequent viability test at a concentration of 50microM. Nerolidol significantly (P<0.05) inhibited the growth of B. microti at the dosage of 10 and 100mg/kg BW, while the inhibition was low compared with the high doses used. Therefore, nerolidol could not be used as a chemotherapeutic drug for babesiosis.

摘要

橙花叔醇是一种存在于许多植物精油中的倍半萜，被美国食品药品监督管理局批准为食品调味剂。橙花叔醇干扰恶性疟原虫顶质体中的类异戊二烯生物合成途径。在本研究中，在橙花叔醇存在的情况下，四种巴贝斯虫物种的体外生长受到显著抑制（P<0.05）（牛巴贝斯虫、双芽巴贝斯虫、卵形巴贝斯虫和马巴贝斯虫的IC50值分别为21±1、29.6±3、26.9±2和23.1±1μM）。来自处理过的培养物中的寄生虫在随后浓度为50μM的活力测试中无法生长。橙花叔醇在剂量为10和100mg/kg体重时显著抑制微小巴贝斯虫的生长，不过与所使用的高剂量相比，抑制作用较低。因此，橙花叔醇不能用作治疗巴贝斯虫病的化疗药物。