评价百里醌对梨形虫寄生虫的体外和体内抑制作用。
Evaluation of the in vitro and in vivo inhibitory effect of thymoquinone on piroplasm parasites.
机构信息
National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-Cho, Obihiro, Hokkaido, Japan.
Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Mansoura University, Mansoura, 35516, Egypt.
出版信息
Parasit Vectors. 2019 Jan 16;12(1):37. doi: 10.1186/s13071-019-3296-z.
BACKGROUND
Developing new antibabesial drugs with a low toxic effect to the animal and with no resistance from Babesia parasites is in urgent demand. In this concern, the antimalarial, anticancer and antioxidant effect of thymoquinone (TQ), a phytochemical compound found in the plant Nigella sativa, has been reported. Therefore, in the present study, the antibabesial effect of this compound was evaluated on the growth of piroplasm parasites.
RESULTS
Significant inhibition (P < 0.05) of the in vitro growth of piroplasm parasites were observed after treatment by TQ with IC values of 35.41 ± 3.60, 7.35 ± 0.17, 0.28 ± 0.016, 74.05 ± 4.55 and 67.33 ± 0.94 μM for Babesia bovis, Babesia bigemina, Babesia divergens, Theileria equi and Babesia caballi, respectively. The in vitro inhibitory effect of TQ was significantly enhanced (P < 0.05) when used in combination with either diminazene aceturate on bovine Babesia and equine Babesia and Theileria cultures. In B. microti-infected mice, oral and intraperitoneal administrations of TQ showed significant (P < 0.05) inhibition of parasite growth at a dose of 70 mg/kg and 50 mg/kg, respectively, compared to the control group.
CONCLUSIONS
The obtained results indicate that thymoquinone might be a promising medicinal compound for use in the treatment of animal piroplasmosis.
背景
开发对动物毒性低且对巴贝斯虫寄生虫无耐药性的新型抗巴贝斯药物迫在眉睫。在这方面，已报道存在于植物黑种草中的植物化学化合物百里醌（TQ）具有抗疟、抗癌和抗氧化作用。因此，在本研究中，评估了该化合物对梨形虫寄生虫生长的抗巴贝斯作用。
结果
用 TQ 处理后，观察到体外滋养体寄生虫的生长受到显著抑制（P < 0.05），IC 值分别为 35.41 ± 3.60、7.35 ± 0.17、0.28 ± 0.016、74.05 ± 4.55 和 67.33 ± 0.94 μM，用于牛巴贝斯虫、双芽巴贝斯虫、分歧巴贝斯虫、马巴贝斯虫和马媾疫巴贝斯虫。当 TQ 与二甲硝咪唑联合用于牛巴贝斯虫和马巴贝斯虫以及泰勒虫培养物时，其体外抑制作用显著增强（P < 0.05）。在感染微小巴贝斯虫的小鼠中，与对照组相比，口服和腹腔内给予 TQ 分别在 70mg/kg 和 50mg/kg 剂量下，寄生虫生长均受到显著抑制（P < 0.05）。
结论
结果表明，百里醌可能是一种有前途的治疗动物梨形虫病的药物。
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