University of Gothenburg, Gothenburg, Sweden.
Chem Soc Rev. 2010 Mar;39(3):985-99. doi: 10.1039/b912918a. Epub 2010 Jan 25.
The power of nanofluidic channels to analyze DNA is described along with practical experimental hints. As an introduction, a general overview is given on conventional DNA analysis tools, as well as tools under development towards the $1000 genome. The focus of this tutorial review is the stretching of DNA in nanoscale channels for coarse-grained mapping of DNA. To understand the behavior of the DNA, basic theory is discussed. Experimental details are revealed so that the reader, with the proper equipment, should be able to perform experiments. Basic approaches to the analysis of the data are discussed. Finally, potential future directions are discussed including the application of melting mapping as a simple barcode for the DNA.
本文描述了纳米通道在分析 DNA 方面的强大功能，并提供了实际的实验提示。作为引言，本文对传统的 DNA 分析工具以及朝着“千元基因组”方向发展的工具进行了概述。本教程综述的重点是在纳米通道中拉伸 DNA，以便对 DNA 进行粗粒度映射。为了理解 DNA 的行为，讨论了基本理论。揭示了实验细节，以便有适当设备的读者能够进行实验。讨论了数据分析的基本方法。最后，讨论了潜在的未来方向，包括将融解映射作为 DNA 的简单条码的应用。
