College of Life Sciences and Biotechnology, Korea University, Anam-Dong, Seongbuk-Gu, Seoul, 136-713, South Korea.
Mol Biol Rep. 2010 Oct;37(7):3567-74. doi: 10.1007/s11033-010-0005-6. Epub 2010 Feb 24.
MicroRNAs (miRNAs) are an abundant class of small regulatory RNAs that regulate the stability and translation of cognate mRNAs. Although an increasing number of porcine miRNAs has recently been identified, the full repertoire of miRNAs in pig remains to be elucidated. To identify porcine miRNAs potentially involved in myogenesis and adipogenesis, we constructed small RNA cDNA libraries from skeletal muscle and adipose tissue and identified 89 distinct miRNAs that are conserved in pig, of which 15 were new. Expression analysis of all newly identified and selected known porcine miRNAs revealed that some miRNAs were enriched in a tissue-specific manner, whereas others were expressed ubiquitously in the porcine tissues examined. Our results expand the number of known porcine miRNAs and provide useful information for further investigating the biological functions of miRNAs associated with growth and development of skeletal muscle or adipose tissue in pig.
MicroRNAs (miRNAs) 是一类丰富的小调控 RNA，可调节同源 mRNA 的稳定性和翻译。尽管最近已经鉴定出越来越多的猪 miRNAs，但猪的完整 miRNA 谱仍有待阐明。为了鉴定可能参与肌发生和脂肪发生的猪 miRNAs，我们从骨骼肌和脂肪组织构建了小 RNA cDNA 文库，并鉴定了 89 种在猪中保守的独特 miRNAs，其中 15 种是新的。对所有新鉴定和选择的已知猪 miRNAs 的表达分析表明，一些 miRNAs 以组织特异性的方式富集，而其他 miRNAs则在检查的猪组织中普遍表达。我们的研究结果扩展了已知的猪 miRNAs 的数量，并为进一步研究与骨骼肌或脂肪组织生长和发育相关的 miRNAs 的生物学功能提供了有用的信息。
