Department of Gastroenterology, Guangzhou First Municipal People's Hospital Affiliated to Guangzhou Medical College, No. 1 Panfu Road, Guangzhou 510180, Guangdong Province, China.
World J Gastroenterol. 2010 Feb 28;16(8):1008-13. doi: 10.3748/wjg.v16.i8.1008.
To investigate the preparation, physicochemical characterization and cytotoxicity in vitro of Gemcitabine-loaded poly(ethylene glycol)-block-poly(D,L-lactide) (PEG-PDLLA) nanovesicles.
The nanovesicle carriers were prepared from the amphiphilic block copolymer of PEG-PDLLA by a double emulsion technique, and gemcitabine was used as the model drug. The morphology of the nanovesicles was determined by scanning and transmission electron microscopy, and the drug content, drug entrapment and drug-release curve in vitro were detected by UV-Vis-NIR spectrophotometry. Cytotoxicity in the human pancreatic cancer cell line SW1990 was tested by 3-(4,5-dimethyl) ethiazole (MTT) assay.
The gemcitabine-loaded nanovesicles were hollow nanospheres with a mean size of 200.6 nm, drug loading of 4.14% and drug embedding ratio of 20.54%. The nanovesicles showed excellent controlled release that was characterized by a fast initial release during the first 72 h, followed by a slower and continuous release. The MTT assay demonstrated that gemcitabine-loaded nanovesicles exhibited dose-dependent and time-delayed cytotoxicity in the human pancreatic cancer cell line SW1990.
Gemcitabine-loaded PEG-PDLLA nanovesicles prepared by a double emulsion technique exhibited good performance for controlled drug release, and had similar cytotoxic activity to free gemcitabine.
研究载吉西他滨的聚乙二醇-嵌段-聚（D，L-丙交酯）（PEG-PDLLA）纳米囊泡的制备、理化性质表征和体外细胞毒性。
采用复乳技术，由两亲性嵌段共聚物 PEG-PDLLA 制备纳米囊泡载体，以吉西他滨为模型药物。通过扫描电子显微镜和透射电子显微镜观察纳米囊泡的形态，通过紫外-可见-近红外分光光度法检测药物含量、包封率和体外药物释放曲线。通过 3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐（MTT）法检测人胰腺癌细胞系 SW1990 的细胞毒性。
载吉西他滨的纳米囊泡为空心纳米球，平均粒径为 200.6nm，载药量为 4.14%，包封率为 20.54%。纳米囊泡具有良好的控制释放性能，在最初的 72 小时内快速释放，随后是较慢的持续释放。MTT 法表明，载吉西他滨的纳米囊泡在人胰腺癌细胞系 SW1990 中表现出剂量依赖性和时间延迟的细胞毒性。
采用复乳技术制备的载吉西他滨的 PEG-PDLLA 纳米囊泡具有良好的控制药物释放性能，其细胞毒性与游离吉西他滨相似。
