TNO Quality of Life, Business Unit Biosciences, Zeist, The Netherlands.
Am J Clin Nutr. 2010 Apr;91(4):1044-59. doi: 10.3945/ajcn.2009.28822. Epub 2010 Feb 24.
Low-grade chronic inflammation in overweight subjects is thought to play an important role in disease development.
It was hypothesized that specific dietary components are able to reduce low-grade inflammation as well as metabolic and oxidative stress.
Dietary products [resveratrol, green tea extract, alpha-tocopherol, vitamin C, n-3 (omega-3) polyunsaturated fatty acids, and tomato extract] selected for their evidence-based antiinflammatory properties were combined and given as supplements to 36 healthy overweight men with mildly elevated plasma C-reactive protein concentrations in a double-blind, placebo-controlled, crossover study with treatment periods of 5 wk. Inflammatory and oxidative stress defense markers were quantified in plasma and urine. Furthermore, 120 plasma proteins, 274 plasma metabolites (lipids, free fatty acids, and polar compounds), and the transcriptomes of peripheral blood mononuclear cells and adipose tissue were quantified.
Plasma adiponectin concentrations increased by 7%, whereas C-reactive protein (principal inflammation marker) was unchanged. However, a multitude of subtle changes were detected by an integrated analysis of the "omics" data, which indicated modulated inflammation of adipose tissue, improved endothelial function, affected oxidative stress, and increased liver fatty acid oxidation.
An intervention with selected dietary products affected inflammatory processes, oxidative stress, and metabolism in humans, as shown by large-scale profiling of genes, proteins, and metabolites in plasma, urine, and adipose tissue. This trial was registered at clinical trials.gov as NCT00655798.
超重人群的低度慢性炎症被认为在疾病发展中起重要作用。
假设特定的膳食成分能够减轻低度炎症以及代谢和氧化应激。
选择具有循证抗炎特性的膳食产品[白藜芦醇、绿茶提取物、α-生育酚、维生素 C、n-3（ω-3）多不饱和脂肪酸和番茄提取物]，将其组合在一起，并作为补充剂给予 36 名超重且血浆 C 反应蛋白浓度轻度升高的健康男性，采用双盲、安慰剂对照、交叉研究，每个治疗期为 5 周。在血浆和尿液中定量测定炎症和氧化应激防御标志物。此外，定量测定了 120 种血浆蛋白、274 种血浆代谢物（脂质、游离脂肪酸和极性化合物）以及外周血单核细胞和脂肪组织的转录组。
血浆脂联素浓度增加 7%，而 C 反应蛋白（主要炎症标志物）不变。然而，通过对“组学”数据的综合分析，发现了大量微妙的变化，这表明脂肪组织炎症得到调节、内皮功能改善、氧化应激受影响以及肝脏脂肪酸氧化增加。
所选膳食产品的干预措施影响了人类的炎症过程、氧化应激和代谢，这可以通过对血浆、尿液和脂肪组织中的基因、蛋白质和代谢物进行大规模分析来证明。本试验在 clinicaltrials.gov 上注册，编号为 NCT00655798。
